BMRBj - BMREntryMaster

Found 1 Document (1.563 seconds)

BMRB: 5485

1GO0

Thermal stability and solution structure of the ribosomal protein L30e from hyperthermophile archaeon Thermococcus celer

Authors

Chan, S., Leung, T., Chen, Y., Bycroft, M., Wong, K.

Assembly

ribosomal protein L30e

Entity

1. ribosomal protein L30e (polymer, Thiol state: not present), 100 monomers, 10835.39 Da Detail

VDFAFELRKA QDTGKIVMGA RKSIQYAKMG GAKLIIVARN ARPDIKEDIE YYARLSGIPV YEFEGTSVEL GTLLGRPHTV SALAVVDPGE SRILALGGKE

Formula weight

10835.39 Da

Source organism

Thermococcus celer

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING USING AMBIGUOUS NOES

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.9 %, Completeness (bb): 84.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.9 % (968 of 1140)	94.9 % (561 of 591)	69.6 % (313 of 450)	94.9 % (94 of 99)
Backbone	84.0 % (497 of 592)	98.1 % (203 of 207)	69.6 % (201 of 289)	96.9 % (93 of 96)
Sidechain	87.6 % (558 of 637)	93.2 % (358 of 384)	79.6 % (199 of 250)	33.3 % (1 of 3)
Aromatic	48.5 % (32 of 66)	97.0 % (32 of 33)	0.0 % (0 of 33)
Methyl	95.4 % (124 of 130)	98.5 % (64 of 65)	92.3 % (60 of 65)

1. Ribosomal protein L30e

VDFAFELRKA QDTGKIVMGA RKSIQYAKMG GAKLIIVARN ARPDIKEDIE YYARLSGIPV YEFEGTSVEL GTLLGRPHTV SALAVVDPGE SRILALGGKE

Show sample condition

Sample

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ribosomal protein L30e			1 mM
2	D20			100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.631 ppm	internal	indirect	0.25145
¹⁵N	water	protons	4.631 ppm	internal	indirect	0.1013291
¹H	water	protons	4.631 ppm	internal	direct	1.0

Referencing offset: 1.85 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.631 ppm	internal	indirect	0.25145
¹⁵N	water	protons	4.631 ppm	internal	indirect	0.1013291
¹H	water	protons	4.631 ppm	internal	direct	1.0

Referencing offset: 1.85 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.631 ppm	internal	indirect	0.25145
¹⁵N	water	protons	4.631 ppm	internal	indirect	0.1013291
¹H	water	protons	4.631 ppm	internal	direct	1.0

Referencing offset: 0.02 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1GO0, Strand ID: A Detail

Release date

2003-07-29

Citation

Solution structure and thermal stability of ribosomal protein L30e from hyperthermophilic archaeon Thermococcus celer
Wong, K., Lee, C., Chan, S., Leung, T., Chen, Y., Bycroft, M.
Protein Sci. (2003), 12, 1483-1495, PubMed 12824494 , DOI 10.1110/ps.0302303 , Abstract

Related entities 1. ribosomal protein L30e, : 1 : 4 : 1 : 7 : 222 entities Detail

Experiments performed 9 experiments Detail

1 2D TOCSY

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ribosomal protein L30e			1 mM
2	D20			100 %

Sample #2

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Ribosomal protein L30e	[U-100% 15N]		1 mM

Sample #3

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM

Sample #4

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM
6	D20			100 %

2 2D NOESY

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ribosomal protein L30e			1 mM
2	D20			100 %

Sample #2

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Ribosomal protein L30e	[U-100% 15N]		1 mM

Sample #3

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM

Sample #4

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM
6	D20			100 %

3 1H-15N HSQC

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ribosomal protein L30e			1 mM
2	D20			100 %

Sample #2

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Ribosomal protein L30e	[U-100% 15N]		1 mM

Sample #3

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM

Sample #4

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM
6	D20			100 %

4 NOESY-HSQC

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ribosomal protein L30e			1 mM
2	D20			100 %

Sample #2

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Ribosomal protein L30e	[U-100% 15N]		1 mM

Sample #3

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM

Sample #4

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM
6	D20			100 %

5 TOCSY-HSQC

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ribosomal protein L30e			1 mM
2	D20			100 %

Sample #2

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Ribosomal protein L30e	[U-100% 15N]		1 mM

Sample #3

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM

Sample #4

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM
6	D20			100 %

6 HNCACB

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ribosomal protein L30e			1 mM
2	D20			100 %

Sample #2

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Ribosomal protein L30e	[U-100% 15N]		1 mM

Sample #3

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM

Sample #4

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM
6	D20			100 %

7 CBCA(CO)NH

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ribosomal protein L30e			1 mM
2	D20			100 %

Sample #2

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Ribosomal protein L30e	[U-100% 15N]		1 mM

Sample #3

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM

Sample #4

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM
6	D20			100 %

8 HNCA

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ribosomal protein L30e			1 mM
2	D20			100 %

Sample #2

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Ribosomal protein L30e	[U-100% 15N]		1 mM

Sample #3

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM

Sample #4

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM
6	D20			100 %

9 1H-13C HCCH-TOCSY

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ribosomal protein L30e			1 mM
2	D20			100 %

Sample #2

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Ribosomal protein L30e	[U-100% 15N]		1 mM

Sample #3

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM

Sample #4

Temperature 310 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Ribosomal protein L30e	[U-100% 15N; U-100% 13C]		1 mM
6	D20			100 %

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Found 1 Document (1.563 seconds)

BMRB: 5485

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. ribosomal protein L30e, : 1 : 4 : 1 : 7 : 222 entities Detail

Experiments performed 9 experiments Detail

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