BMRB: 5491

Structural basis for new pattern of the conserved amino acid residues related to chitin-binding in the antifungal peptide from the coconut rhinoceros beetle, Oryctes rhinoceros

Authors

Hemmi, H., Ishibashi, J., Tomie, T., Yamakawa, M.

Assembly

scarabaecin monomer

Entity

1. scarabaecin monomer (polymer, Thiol state: all disulfide bound), 36 monomers, 4080.688 Da Detail

ELPKLPDDKV LIRSRSNCPK GKVWNGFDCK SPFAFS

Formula weight

4080.688 Da

Entity Connection

disulfide 1 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS18:SG	1:CYS29:SG

Source organism

Oryctes rhinoceros

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 68.6 %, Completeness (bb): 64.2 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C
All	68.6 % (277 of 404)	99.6 % (233 of 234)	25.9 % (44 of 170)
Backbone	64.2 % (113 of 176)	98.6 % (69 of 70)	41.5 % (44 of 106)
Sidechain	64.5 % (169 of 262)	100.0 % (164 of 164)	5.1 % (5 of 98)
Aromatic	51.2 % (21 of 41)	100.0 % (21 of 21)	0.0 % (0 of 20)
Methyl	53.8 % (14 of 26)	100.0 % (13 of 13)	7.7 % (1 of 13)

1. scarabaecin

ELPKLPDDKV LIRSRSNCPK GKVWNGFDCK SPFAFS

Show sample condition

Sample

Temperature 298 (±0.01) K, pH 2.4 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	scarabaecin			3 mM

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 1IYC, Strand ID: A Detail

---

Release date

2002-08-04

Citation

Structural basis for new pattern of conserved amino acid residues related to chitin-binding in the antifungal peptide from the coconut rhinoceros beetle Oryctes rhinoceros
Hemmi, H., Ishibashi, J., Tomie, T., Yamakawa, M.
J. Biol. Chem. (2003), 278, 22820-22827, PubMed 12676931 , DOI 10.1074/jbc.M301025200 , Abstract

Related entities 1. scarabaecin monomer, : 1 : 1 entities Detail

Experiments performed 5 experiments Detail