BMRB: 5494

NMR Structure of PW2 Bound to SDS Micelles: A Tryptophan-rich Anticocidial Peptide Selected from Phage Display Libraries

Authors

Tinoco, L.W., da Silva, A., Leite, A., Valente, A.P., Almeida, F.C.

Assembly

PW2 Bound to SDS Micelles

Entity

1. PW2 Bound to SDS Micelles (polymer, Thiol state: not present), 12 monomers, 1610.856 Da Detail

HPLKQYWWRP SI

Formula weight

1610.856 Da

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 82.8 %, Completeness (bb): 100.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	82.8 % (77 of 93)	82.8 % (77 of 93)
Backbone	100.0 % (22 of 22)	100.0 % (22 of 22)
Sidechain	77.5 % (55 of 71)	77.5 % (55 of 71)
Aromatic	44.4 % (8 of 18)	44.4 % (8 of 18)
Methyl	25.0 % (1 of 4)	25.0 % (1 of 4)

1. anticocidial peptide

HPLKQYWWRP SI

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	anticocidial peptide			4.0 mM
2	sodium_phosphate_buffer			20.0 mM
3	sodium_chloride			100.0 mM
4	D2O			10 %

Hide sample codition

Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 90.9 %, Completeness (bb): 100.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	90.9 % (169 of 186)	90.9 % (169 of 186)
Backbone	100.0 % (44 of 44)	100.0 % (44 of 44)
Sidechain	88.0 % (125 of 142)	88.0 % (125 of 142)
Aromatic	72.2 % (26 of 36)	72.2 % (26 of 36)
Methyl	50.0 % (4 of 8)	50.0 % (4 of 8)

1. anticocidial peptide

HPLKQYWWRP SI

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	anticocidial peptide			4.0 mM
6	sodium_phosphate_buffer			20.0 mM
7	SDS			40.0 mM
8	sodium_chloride			100.0 mM

Hide sample codition

Chem. Shift Complete3

Sequence coverage: 100.0 %, Completeness: 88.5 %, Completeness (bb): 100.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	88.5 % (247 of 279)	88.5 % (247 of 279)
Backbone	100.0 % (66 of 66)	100.0 % (66 of 66)
Sidechain	85.0 % (181 of 213)	85.0 % (181 of 213)
Aromatic	75.9 % (41 of 54)	75.9 % (41 of 54)
Methyl	41.7 % (5 of 12)	41.7 % (5 of 12)

1. anticocidial peptide

HPLKQYWWRP SI

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
9	anticocidial peptide			4.0 mM
10	sodium_phosphate_buffer			20.0 mM
11	sodium_chloride			100.0 mM
12	SDS			160.0 mM
13	D2O			10.0 %

Hide sample codition

Protein Blocks Logo

Calculated from 19 models in PDB: 1M02, Strand ID: A Detail

---

Release date

2002-08-21

Citation

NMR structure of PW2 bound to SDS micelles. A tryptophan-rich anticoccidial peptide selected from phage display libraries
Tinoco, L.W., da Silva, A., Leite, A., Valente, A.P., Almeida, F.C.
J. Biol. Chem. (2002), 277, 36351-36356, PubMed 12130641 , DOI 10.1074/jbc.M204225200 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. PW2 Bound to SDS Micelles, : 1 : 1 : 2 entities Detail

Experiments performed 2 experiments Detail