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Found 1 Document (1.834 seconds)

BMRB: 5499

1M5Z

The PDZ7 of Glutamate Receptor Interacting Protein Binds to its Target via a Novel Hydrophobic Surface Area

Authors

Feng, W., Fan, J., Jiang, M., Shi, Y., Zhang, M.

Assembly

AMPA receptor interacting protein

Entity

1. AMPA receptor interacting protein (polymer, Thiol state: all free), 91 monomers, 9966.347 Da Detail

SPTPVELHKV TLYKDSGMED FGFSVADGLL EKGVYVKNIR PAGPGDLGGL KPYDRLLQVN HVRTRDFDCC LVVPLIAESG NKLDLVISRN P

Formula weight

9966.347 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 82.9 %, Completeness (bb): 88.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.9 % (865 of 1044)	82.9 % (450 of 543)	82.8 % (341 of 412)	83.1 % (74 of 89)
Backbone	88.9 % (473 of 532)	89.7 % (165 of 184)	89.0 % (235 of 264)	86.9 % (73 of 84)
Sidechain	78.5 % (466 of 594)	79.4 % (285 of 359)	78.7 % (181 of 230)	0.0 % (0 of 5)
Aromatic	32.3 % (20 of 62)	51.6 % (16 of 31)	12.9 % (4 of 31)
Methyl	91.1 % (102 of 112)	96.4 % (54 of 56)	85.7 % (48 of 56)

1. the seventh PDZ domain of Glutamate Receptor Interacting Protein 1

SPTPVELHKV TLYKDSGMED FGFSVADGLL EKGVYVKNIR PAGPGDLGGL KPYDRLLQVN HVRTRDFDCC LVVPLIAESG NKLDLVISRN P

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Concentration
1	the seventh PDZ domain of Glutamate Receptor Interacting Protein 1	1.0 mM
2	D2O	99.9 %
3	potassium phosphate buffer	100 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	the seventh PDZ domain of Glutamate Receptor Interacting Protein 1	[U-100% 15N]	1 mM
5	H2O		90 %
6	D20		10 %
7	potassium phosphate buffer		100 mM

Sample #3

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	the seventh PDZ domain of Glutamate Receptor Interacting Protein 1	[U-100% 15N;U-100% 13C]	1.0 mM
9	D2O		99.9 %
10	potassium phosphate buffer		100 mM

Sample #4

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
11	the seventh PDZ domain of Glutamate Receptor Interacting Protein 1	[U-100% 15N; U-100% 13C]	1.0 mM
12	H2O		90 %
13	D2O		10 %
14	potassium phosphate buffer		100 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.87 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.87 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.04 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1M5Z, Strand ID: A Detail

Release date

2003-02-19

Citation

PDZ7 of glutamate receptor interacting protein binds to its target via a novel hydrophobic surface area
Feng, W., Fan, J., Jiang, M., Shi, Y., Zhang, M.
J. Biol. Chem. (2002), 277, 41140-41146, PubMed 12196542 , DOI 10.1074/jbc.M207206200 , Abstract

Related entities 1. AMPA receptor interacting protein, : 1 : 2 : 2 : 2 : 238 entities Detail

Interaction partners 1. AMPA receptor interacting protein, : 27 interactors Detail

More details for 27 interactors identified by 10 citations

Experiments performed 3 experiments Detail

1 2D NOESY

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Concentration
1	the seventh PDZ domain of Glutamate Receptor Interacting Protein 1	1.0 mM
2	D2O	99.9 %
3	potassium phosphate buffer	100 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	the seventh PDZ domain of Glutamate Receptor Interacting Protein 1	[U-100% 15N]	1 mM
5	H2O		90 %
6	D20		10 %
7	potassium phosphate buffer		100 mM

Sample #3

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	the seventh PDZ domain of Glutamate Receptor Interacting Protein 1	[U-100% 15N;U-100% 13C]	1.0 mM
9	D2O		99.9 %
10	potassium phosphate buffer		100 mM

Sample #4

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
11	the seventh PDZ domain of Glutamate Receptor Interacting Protein 1	[U-100% 15N; U-100% 13C]	1.0 mM
12	H2O		90 %
13	D2O		10 %
14	potassium phosphate buffer		100 mM

2 3D 15N-separated_NOESY

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Concentration
1	the seventh PDZ domain of Glutamate Receptor Interacting Protein 1	1.0 mM
2	D2O	99.9 %
3	potassium phosphate buffer	100 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	the seventh PDZ domain of Glutamate Receptor Interacting Protein 1	[U-100% 15N]	1 mM
5	H2O		90 %
6	D20		10 %
7	potassium phosphate buffer		100 mM

Sample #3

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	the seventh PDZ domain of Glutamate Receptor Interacting Protein 1	[U-100% 15N;U-100% 13C]	1.0 mM
9	D2O		99.9 %
10	potassium phosphate buffer		100 mM

Sample #4

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
11	the seventh PDZ domain of Glutamate Receptor Interacting Protein 1	[U-100% 15N; U-100% 13C]	1.0 mM
12	H2O		90 %
13	D2O		10 %
14	potassium phosphate buffer		100 mM

3 3D 13C-separated_NOESY

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Concentration
1	the seventh PDZ domain of Glutamate Receptor Interacting Protein 1	1.0 mM
2	D2O	99.9 %
3	potassium phosphate buffer	100 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	the seventh PDZ domain of Glutamate Receptor Interacting Protein 1	[U-100% 15N]	1 mM
5	H2O		90 %
6	D20		10 %
7	potassium phosphate buffer		100 mM

Sample #3

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	the seventh PDZ domain of Glutamate Receptor Interacting Protein 1	[U-100% 15N;U-100% 13C]	1.0 mM
9	D2O		99.9 %
10	potassium phosphate buffer		100 mM

Sample #4

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
11	the seventh PDZ domain of Glutamate Receptor Interacting Protein 1	[U-100% 15N; U-100% 13C]	1.0 mM
12	H2O		90 %
13	D2O		10 %
14	potassium phosphate buffer		100 mM

nullKeywords PDZ domain, Glutamate receptor interacting protein 1, protein structure, protein interaction

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Found 1 Document (1.834 seconds)

BMRB: 5499

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Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. AMPA receptor interacting protein, : 1 : 2 : 2 : 2 : 238 entities Detail

Interaction partners 1. AMPA receptor interacting protein, : 27 interactors Detail

Experiments performed 3 experiments Detail

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Sample #1

Sample #2

Sample #3

Sample #4

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Sample #4

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Sample #4