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BMRB: 5500

1IYF

Parkin binds the Rpn10 subunit of 26S proteasomes with the ubiquitin-like domain

Authors

Sakata, E., Yamaguchi, Y., Kurimoto, E., Kikuchi, J., Yokoyama, S., Kawahara, H., Yokosawa, H., Hattori, N., Mizuno, Y., Tanaka, K., Kato, K.

Assembly

parkin ubiquitin-like domain

Entity

1. parkin ubiquitin-like domain (polymer, Thiol state: all free), 81 monomers, 9235.437 Da Detail

GPLGSMIVFV RFNSSHGFPV EVDSDTSIFQ LKEVVAKRQG VPADQLRVIF AGKELRNDWT VQNCDLDQQS IVHIVQRPWR K

Formula weight

9235.437 Da

Source organism

Exptl. method

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.8 %, Completeness: 45.4 %, Completeness (bb): 91.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	45.4 % (441 of 971)	30.2 % (153 of 507)	57.6 % (216 of 375)	80.9 % (72 of 89)
Backbone	91.2 % (436 of 478)	90.8 % (148 of 163)	90.8 % (216 of 238)	93.5 % (72 of 77)
Sidechain	13.0 % (74 of 569)	1.5 % (5 of 344)	32.4 % (69 of 213)	0.0 % (0 of 12)
Aromatic	0.0 % (0 of 82)	0.0 % (0 of 41)	0.0 % (0 of 39)	0.0 % (0 of 2)
Methyl	5.3 % (5 of 94)	4.3 % (2 of 47)	6.4 % (3 of 47)

1. Parkin ubiquitin-like domain

GPLGSMIVFV RFNSSHGFPV EVDSDTSIFQ LKEVVAKRQG VPADQLRVIF AGKELRNDWT VQNCDLDQQS IVHIVQRPWR K

Show sample condition

Sample

Temperature 303 (±2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Parkin ubiquitin-like domain	[U-98% 13C; U-98% 15N]		0.1 mM
2	potasium phosphate buffer			50 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 1IYF, Strand ID: A Detail

Release date

2003-03-09

Citation

Parkin binds the Rpn10 subunit of 26S proteasomes through its ubiquitin-like domain
Sakata, E., Yamaguchi, Y., Kurimoto, E., Kikuchi, J., Yokoyama, S., Kawahara, H., Yokosawa, H., Hattori, N., Mizuno, Y., Tanaka, K., Kato, K.
EMBO Rep. (2003), 4, 301-306, PubMed 12634850 , DOI 10.1038/sj.embor.embor764 , Abstract

Related entities 1. parkin ubiquitin-like domain, : 1 : 8 : 641 entities Detail

Interaction partners 1. parkin ubiquitin-like domain, : 39 interactors Detail

More details for 39 interactors identified by 21 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 303 (±2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Parkin ubiquitin-like domain	[U-98% 13C; U-98% 15N]		0.1 mM
2	potasium phosphate buffer			50 mM

2 2D 1H-13C HSQC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 303 (±2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Parkin ubiquitin-like domain	[U-98% 13C; U-98% 15N]		0.1 mM
2	potasium phosphate buffer			50 mM

3 3D CBCA(CO)NH

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 303 (±2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Parkin ubiquitin-like domain	[U-98% 13C; U-98% 15N]		0.1 mM
2	potasium phosphate buffer			50 mM

4 3D CBCANH

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 303 (±2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Parkin ubiquitin-like domain	[U-98% 13C; U-98% 15N]		0.1 mM
2	potasium phosphate buffer			50 mM

5 3D HCACO

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 303 (±2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Parkin ubiquitin-like domain	[U-98% 13C; U-98% 15N]		0.1 mM
2	potasium phosphate buffer			50 mM

6 3D HNCA

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 303 (±2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Parkin ubiquitin-like domain	[U-98% 13C; U-98% 15N]		0.1 mM
2	potasium phosphate buffer			50 mM

7 3D HN(CO)CA

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 303 (±2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Parkin ubiquitin-like domain	[U-98% 13C; U-98% 15N]		0.1 mM
2	potasium phosphate buffer			50 mM

8 3D HNCO

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 303 (±2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Parkin ubiquitin-like domain	[U-98% 13C; U-98% 15N]		0.1 mM
2	potasium phosphate buffer			50 mM

9 3D 15N-edited TOCSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 303 (±2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Parkin ubiquitin-like domain	[U-98% 13C; U-98% 15N]		0.1 mM
2	potasium phosphate buffer			50 mM

nullKeywords parkin, ubiquitin-like domain, proteasome