BMRB: 5501

The Solution Structure of Human Hepcidin, a Peptide-Hormone with Antimicrobial Activity that is Involved in Iron Uptake and Hereditary Hemochromatosis

Authors

Hunter, H.N., Fulton, D.B., Ganz, T., Vogel, H.J.

Assembly

Hepcidin

Entity

1. Hepcidin (polymer, Thiol state: all disulfide bound), 25 monomers, 2797.414 Da Detail

DTHFPICIFC CGCCHRSKCG MCCKT

Formula weight

2797.414 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS23:SG
2	disulfide	sing	1:CYS10:SG	1:CYS22:SG
3	disulfide	sing	1:CYS11:SG	1:CYS19:SG
4	disulfide	sing	1:CYS13:SG	1:CYS14:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 80.1 %, Completeness (bb): 51.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	80.1 % (113 of 141)	80.1 % (113 of 141)
Backbone	51.0 % (26 of 51)	51.0 % (26 of 51)
Sidechain	96.7 % (87 of 90)	96.7 % (87 of 90)
Aromatic	100.0 % (14 of 14)	100.0 % (14 of 14)
Methyl	100.0 % (6 of 6)	100.0 % (6 of 6)

1. hepicidin

DTHFPICIFC CGCCHRSKCG MCCKT

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 3.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hepicidin	[U-15N]-Gly,Phe,Ile		1.6 mM
2	phosphate buffer			40 mM
3	H2O			90 %
4	D2O			10 %

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Calculated from 20 models in PDB: 1M4F, Strand ID: A Detail

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Release date

2002-08-13

Citation

The solution structure of human hepcidin, a peptide hormone with antimicrobial activity that is involved in iron uptake and hereditary hemochromatosis
Hunter, H.N., Fulton, D.B., Ganz, T., Vogel, H.J.
J. Biol. Chem. (2002), 277, 37597-37603, PubMed 12138110 , DOI 10.1074/jbc.M205305200 , Abstract

Related entities 1. Hepcidin, : 1 : 7 : 1 : 22 entities Detail

Interaction partners 1. Hepcidin, : 1 interactors Detail

Experiments performed 3 experiments Detail