BMRB: 5503

1H resonance assignment of the C-terminal domain of Human Centrin2 (LC-HsCen2: M84-Y172)

Authors

Matei, E., Miron, S., Blouquit, Y., Duchambon, P., Durussel, P., Cox, J.A., Craescu, C.T.

Assembly

Human centrin2

Entity

1. Human centrin2 (polymer, Thiol state: not present), 89 monomers, 10376.57 Da Detail

MNFGDFLTVM TQKMSEKDTK EEILKAFKLF DDDETGKISF KNLKRVAKEL GENLTDEELQ EMIDEADRDG DGEVSEQEFL RIMKKTSLY

Formula weight

10376.57 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry, restraint energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.9 %, Completeness: 82.3 %, Completeness (bb): 88.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	82.3 % (464 of 564)	82.3 % (464 of 564)
Backbone	88.5 % (162 of 183)	88.5 % (162 of 183)
Sidechain	79.3 % (302 of 381)	79.3 % (302 of 381)
Aromatic	97.1 % (33 of 34)	97.1 % (33 of 34)
Methyl	97.6 % (40 of 41)	97.6 % (40 of 41)

1. Human centrin2

MNFGDFLTVM TQKMSEKDTK EEILKAFKLF DDDETGKISF KNLKRVAKEL GENLTDEELQ EMIDEADRDG DGEVSEQEFL RIMKKTSLY

Show sample condition

Sample

Pressure 1 atm, Temperature 308 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Human centrin2			1.5 mM
2	Tris_d11	[U-2H]		20 mM
3	NaCl			100 mM
4	H2O			95 %
5	D2O			5 %

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Protein Blocks Logo

Calculated from 25 models in PDB: 1M39, Strand ID: A Detail

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Release date

2003-03-13

Citation

C-terminal half of human centrin 2 behaves like a regulatory EF-hand domain
Matei, E., Miron, S., Blouquit, Y., Duchambon, P., Durussel, P., Cox, J.A., Craescu, C.T.
Biochemistry (2003), 42, 1439-1450, PubMed 12578356 , DOI 10.1021/bi0269714 , Abstract

Related entities 1. Human centrin2, : 1 : 2 : 4 : 380 entities Detail

Interaction partners 1. Human centrin2, : 23 interactors Detail

Experiments performed 3 experiments Detail