BMRB: 5511

Backbone 1H, 13C, and 15N resonance assignments for the 21 kDa GTPase Rac1 complexed to GDP and Mg2+

Authors

Thapar, R., Moore, C.D., Campbell, S.L.

Assembly

p21 Rac1 GTPase

Entity

1. Rac1 (polymer, Thiol state: all free), 188 monomers, 20991.17 Da Detail

MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE VRHHCPNTPI ILVGTKLDLR DDKDTIEKLK EKKLTPITYP QGLAMAKEIG AVKYLECSAL TQRGLKTVFD EAIRAVLSPP PVKKRKRK

2. GDP (non-polymer), 443.201 Da
3. MG (non-polymer), 24.305 Da

Total weight

21458.676 Da

Max. entity weight

20991.17 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	2:GDP1:O2G	3:MG1:MG
2	na	sing	2:GDP1:O2B	3:MG1:MG
3	na	sing	3:MG1:MG	1:THR17:OG1
4	na	sing	3:MG1:MG	1:THR35:OG1

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 73.9 %, Completeness: 28.3 %, Completeness (bb): 51.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	28.3 % (629 of 2219)	16.7 % (193 of 1155)	34.0 % (298 of 877)	73.8 % (138 of 187)
Backbone	51.5 % (566 of 1100)	39.9 % (149 of 373)	50.5 % (279 of 553)	79.3 % (138 of 174)
Sidechain	6.0 % (78 of 1296)	5.6 % (44 of 782)	6.8 % (34 of 501)	0.0 % (0 of 13)
Aromatic	0.0 % (0 of 156)	0.0 % (0 of 78)	0.0 % (0 of 76)	0.0 % (0 of 2)
Methyl	7.3 % (17 of 232)	6.9 % (8 of 116)	7.8 % (9 of 116)

1. Rac1

MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE VRHHCPNTPI ILVGTKLDLR DDKDTIEKLK EKKLTPITYP QGLAMAKEIG AVKYLECSAL TQRGLKTVFD EAIRAVLSPP PVKKRKRK

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Sample

Temperature 298 (±1.5) K, pH 6.8 (±0.2), Details The sample is uniformly labeled with 15N and 13C and is perdeuterated

#	Name	Isotope labeling	Type	Concentration
1	Rac1	[U-15N; U-13C; U-2H]		0.5 ~ 0.7 mM

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Protein Blocks Logo

Calculated from 1 models in PDB: 1FOE, Strand ID: B, D, F, H Detail

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Release date

2005-11-13

Citation

Letter to the Editor: Backbone 1H, 13C, and 15N resonance assignments for the 21 kDa GTPase Rac1 complexed to GDP and Mg2+
Thapar, R., Moore, C.D., Campbell, S.L.
J. Biomol. NMR (2003), 27, 87-88

Related entities 1. Rac1, : 1 : 60 : 240 entities Detail

Interaction partners 1. Rac1, : 5 interactors Detail

Experiments performed 9 experiments Detail