BMRBj - BMREntryMaster

Found 1 Document (1.877 seconds)

BMRB: 5525

1M9O

NMR structure of the first Zinc Binding domain of Nup475/TTP/TIS11

Authors

Amann, B.T., Guerrerio, A.L., Pelo, J., Luo, R.Q., Worthington, M.T., Berg, J.M.

Assembly

Tristetraproline

Entity

1. Tristetraproline (polymer, Thiol state: free and other bound), 77 monomers, 8952.029 Da Detail

GSHMTTSSRY KTELCRTYSE SGRCRYGAKC QFAHGLGELR QANRHPKYKT ELCHKFKLQG RCPYGSRCHF IHNPTED

2. ZN (non-polymer), 65.409 Da

Total weight

9017.438 Da

Max. entity weight

8952.029 Da

Entity Connection

na 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	2:ZN1:ZN	1:CYS15:SG
2	na	sing	2:ZN1:ZN	1:CYS24:SG
3	na	sing	2:ZN1:ZN	1:CYS30:SG

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

CNS, dynamical annealing protocal with molecular dynamic scheme of torsion,torsion,cartesian.

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete1

Sequence coverage: 51.9 %, Completeness: 33.2 %, Completeness (bb): 40.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C
All	33.2 % (273 of 822)	41.5 % (198 of 477)	21.7 % (75 of 345)
Backbone	40.6 % (155 of 382)	50.6 % (80 of 158)	33.5 % (75 of 224)
Sidechain	30.0 % (153 of 510)	37.0 % (118 of 319)	18.3 % (35 of 191)
Aromatic	11.7 % (11 of 94)	23.4 % (11 of 47)	0.0 % (0 of 47)
Methyl	33.3 % (14 of 42)	52.4 % (11 of 21)	14.3 % (3 of 21)

1. Tristetraproline

GSHMTTSSRY KTELCRTYSE SGRCRYGAKC QFAHGLGELR QANRHPKYKT ELCHKFKLQG RCPYGSRCHF IHNPTED

Show sample condition

Sample

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Tristetraproline		1.7 mM
2	ZnCl2		3.74 mM
3	Tris	[U-2H]	50 mM
4	H20		90 %
5	D20		10 %

Chem. Shift Complete2

Sequence coverage: 7.8 %, Completeness: 20.1 %, Completeness (bb): 23.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C
All	20.1 % (330 of 1644)	24.6 % (235 of 954)	13.8 % (95 of 690)
Backbone	23.2 % (177 of 764)	28.8 % (91 of 316)	19.2 % (86 of 448)
Sidechain	18.9 % (193 of 1020)	22.6 % (144 of 638)	12.8 % (49 of 382)
Aromatic	10.1 % (19 of 188)	16.0 % (15 of 94)	4.3 % (4 of 94)
Methyl	20.2 % (17 of 84)	31.0 % (13 of 42)	9.5 % (4 of 42)

1. Tristetraproline

GSHMTTSSRY KTELCRTYSE SGRCRYGAKC QFAHGLGELR QANRHPKYKT ELCHKFKLQG RCPYGSRCHF IHNPTED

Show sample condition

Sample

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Tristetraproline		1.7 mM
2	ZnCl2		3.74 mM
3	Tris	[U-2H]	50 mM
4	H20		90 %
5	D20		10 %

Protein Blocks Logo

Calculated from 23 models in PDB: 1M9O, Strand ID: A Detail

Coupling constant

15 J values in 1 lists
Pressure 1 atm, Temperature 293 K, pH 5.8 Detail

Release date

2003-09-04

Citation

A Cys3His zinc-binding domain from Nup475/tristetraprolin: a novel fold with a disklike structure
Amann, B.T., Worthington, M.T., Berg, J.M.
Biochemistry (2003), 42, 217-221, PubMed 12515557 , DOI 10.1021/bi026988m , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P22893 released on 1991-08-01
Title TTP_MOUSE Entity Tristetraprolin

Related entities 1. Tristetraproline, : 1 : 4 : 143 entities Detail

Interaction partners 1. Tristetraproline, : 6 interactors Detail

More details for 6 interactors identified by 4 citations

Experiments performed 9 experiments Detail

1 2D NOESY

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Tristetraproline		1.7 mM
2	ZnCl2		3.74 mM
3	Tris	[U-2H]	50 mM
4	H20		90 %
5	D20		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
6	Tristetraproline		1.7 mM
7	ZnCl2		3.74 mM
8	Tris	[U-2H]	50 mM
9	D20		100 %

2 2D TOCSY

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Tristetraproline		1.7 mM
2	ZnCl2		3.74 mM
3	Tris	[U-2H]	50 mM
4	H20		90 %
5	D20		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
6	Tristetraproline		1.7 mM
7	ZnCl2		3.74 mM
8	Tris	[U-2H]	50 mM
9	D20		100 %

3 DQF-COSY

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Tristetraproline		1.7 mM
2	ZnCl2		3.74 mM
3	Tris	[U-2H]	50 mM
4	H20		90 %
5	D20		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
6	Tristetraproline		1.7 mM
7	ZnCl2		3.74 mM
8	Tris	[U-2H]	50 mM
9	D20		100 %

4 3D 13C-separated NOESY

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Tristetraproline		1.7 mM
2	ZnCl2		3.74 mM
3	Tris	[U-2H]	50 mM
4	H20		90 %
5	D20		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
6	Tristetraproline		1.7 mM
7	ZnCl2		3.74 mM
8	Tris	[U-2H]	50 mM
9	D20		100 %

5 3D 15N-separated NOESY

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Tristetraproline		1.7 mM
2	ZnCl2		3.74 mM
3	Tris	[U-2H]	50 mM
4	H20		90 %
5	D20		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
6	Tristetraproline		1.7 mM
7	ZnCl2		3.74 mM
8	Tris	[U-2H]	50 mM
9	D20		100 %

6 HNCACB

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Tristetraproline		1.7 mM
2	ZnCl2		3.74 mM
3	Tris	[U-2H]	50 mM
4	H20		90 %
5	D20		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
6	Tristetraproline		1.7 mM
7	ZnCl2		3.74 mM
8	Tris	[U-2H]	50 mM
9	D20		100 %

7 15N-HSQC-TOCSY

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Tristetraproline		1.7 mM
2	ZnCl2		3.74 mM
3	Tris	[U-2H]	50 mM
4	H20		90 %
5	D20		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
6	Tristetraproline		1.7 mM
7	ZnCl2		3.74 mM
8	Tris	[U-2H]	50 mM
9	D20		100 %

8 13C-HCCH-TOCSY

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Tristetraproline		1.7 mM
2	ZnCl2		3.74 mM
3	Tris	[U-2H]	50 mM
4	H20		90 %
5	D20		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
6	Tristetraproline		1.7 mM
7	ZnCl2		3.74 mM
8	Tris	[U-2H]	50 mM
9	D20		100 %

9 HSQCJ WEX

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Tristetraproline		1.7 mM
2	ZnCl2		3.74 mM
3	Tris	[U-2H]	50 mM
4	H20		90 %
5	D20		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.8

#	Name	Isotope labeling	Concentration
6	Tristetraproline		1.7 mM
7	ZnCl2		3.74 mM
8	Tris	[U-2H]	50 mM
9	D20		100 %

nullKeywords Cys3His type zinc finger

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Found 1 Document (1.877 seconds)

BMRB: 5525

Sample

Sample

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Tristetraproline, : 1 : 4 : 143 entities Detail

Interaction partners 1. Tristetraproline, : 6 interactors Detail

Experiments performed 9 experiments Detail

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