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BMRB: 5528

1M82

Solution structure of the complementary RNA promoter of influenza a virus

Authors

Park, C., Bae, S., Varani, G., Lee, M., Choi, B.

Assembly

RNA (25-MER):the complementary RNA promoter of influenza a virus

Entity

1. RNA (25-MER):the complementary RNA promoter of influenza a virus (polymer, Thiol state: not present), 25 monomers, 8046.710 Da Detail

GGAAGCAGGC UUCGGCCUUG UUUCC

Formula weight

8046.71 Da

Source organism

Influenza A virus

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 52.0 %, Completeness: 52.0 %, Completeness (bb): 52.0 % Detail

Polymer type: polyribonucleotide

	Total	³¹P
All	52.0 % (13 of 25)	52.0 % (13 of 25)
Suger, PO4	52.0 % (13 of 25)	52.0 % (13 of 25)

1. complementary RNA promoter of influenza virus

GGAAGCAGGC UUCGGCCUUG UUUCC

Show sample condition

Sample #1

Pressure 1 atm, Temperature 300 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	complementary RNA promoter of influenza virus			1 mM
2	phosphate buffer			20 mM
3	EDTA			0.1 mM
4	H2O			90 %
5	D2O			10 %

Sample #2

Pressure 1 atm, Temperature 300 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	complementary RNA promoter of influenza virus	[U-15N; U-13C]		1 mM
7	phosphate buffer			20 mM
8	EDTA			0.1 mM
9	D2O			100 %

Release date

2003-07-06

Citation

Solution structure of the influenza A virus cRNA promoter: implications for differential recognition of viral promoter structures by RNA-dependent RNA polymerase
Park, C., Bae, S., Lee, M., Varani, G., Choi, B.
Nucleic Acids Res. (2003), 31, 2824-2832, PubMed 12771209 , Abstract

Related entities 1. RNA (25-MER):the complementary RNA promoter of influenza a virus, : 1 entities Detail

Experiments performed 7 experiments Detail

1 2D NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	complementary RNA promoter of influenza virus			1 mM
2	phosphate buffer			20 mM
3	EDTA			0.1 mM
4	H2O			90 %
5	D2O			10 %

Sample #2

Pressure 1 atm, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	complementary RNA promoter of influenza virus	[U-15N; U-13C]		1 mM
7	phosphate buffer			20 mM
8	EDTA			0.1 mM
9	D2O			100 %

2 2D TOCSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	complementary RNA promoter of influenza virus			1 mM
2	phosphate buffer			20 mM
3	EDTA			0.1 mM
4	H2O			90 %
5	D2O			10 %

Sample #2

Pressure 1 atm, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	complementary RNA promoter of influenza virus	[U-15N; U-13C]		1 mM
7	phosphate buffer			20 mM
8	EDTA			0.1 mM
9	D2O			100 %

3 DQF-COSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	complementary RNA promoter of influenza virus			1 mM
2	phosphate buffer			20 mM
3	EDTA			0.1 mM
4	H2O			90 %
5	D2O			10 %

Sample #2

Pressure 1 atm, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	complementary RNA promoter of influenza virus	[U-15N; U-13C]		1 mM
7	phosphate buffer			20 mM
8	EDTA			0.1 mM
9	D2O			100 %

4 HETCOR

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	complementary RNA promoter of influenza virus			1 mM
2	phosphate buffer			20 mM
3	EDTA			0.1 mM
4	H2O			90 %
5	D2O			10 %

Sample #2

Pressure 1 atm, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	complementary RNA promoter of influenza virus	[U-15N; U-13C]		1 mM
7	phosphate buffer			20 mM
8	EDTA			0.1 mM
9	D2O			100 %

5 3D 13C NOESY-HSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	complementary RNA promoter of influenza virus			1 mM
2	phosphate buffer			20 mM
3	EDTA			0.1 mM
4	H2O			90 %
5	D2O			10 %

Sample #2

Pressure 1 atm, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	complementary RNA promoter of influenza virus	[U-15N; U-13C]		1 mM
7	phosphate buffer			20 mM
8	EDTA			0.1 mM
9	D2O			100 %

6 3D HCCH-COSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	complementary RNA promoter of influenza virus			1 mM
2	phosphate buffer			20 mM
3	EDTA			0.1 mM
4	H2O			90 %
5	D2O			10 %

Sample #2

Pressure 1 atm, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	complementary RNA promoter of influenza virus	[U-15N; U-13C]		1 mM
7	phosphate buffer			20 mM
8	EDTA			0.1 mM
9	D2O			100 %

7 3D HCCH-COSY-TOCSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	complementary RNA promoter of influenza virus			1 mM
2	phosphate buffer			20 mM
3	EDTA			0.1 mM
4	H2O			90 %
5	D2O			10 %

Sample #2

Pressure 1 atm, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	complementary RNA promoter of influenza virus	[U-15N; U-13C]		1 mM
7	phosphate buffer			20 mM
8	EDTA			0.1 mM
9	D2O			100 %

nullKeywords A-form helix, asymmetric internal loop