BMRB: 5530

Naturally occurring modifications reduces the anticodon domain conformational space of tRNA-PHE

Authors

Stuart, J.W., Koshlap, K.M., Guenther, R.H., Agris, P.F.

Assembly

5'-R(*CP*CP*AP*GP*AP*(OMC)P*UP*(OMG)P*AP*AP*GP*AP*UP*(5MC)P*UP*GP*G)-3'

Entity

1. 5'-R(*CP*CP*AP*GP*AP*(OMC)P*UP*(OMG)P*AP*AP*GP*AP*UP*(5MC)P*UP*GP*G)-3' (polymer, Thiol state: not present), 17 monomers, 5571.375 Da Detail

CCAGAXUXAA GAUXUGG

Formula weight

5571.375 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 82.4 %, Completeness: 77.3 %, Completeness (bb): 70.2 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	77.3 % (92 of 119)	77.3 % (92 of 119)
Suger, PO4	70.2 % (59 of 84)	70.2 % (59 of 84)
Nucleobase	94.3 % (33 of 35)	94.3 % (33 of 35)
Aromatic	93.5 % (29 of 31)	93.5 % (29 of 31)

1. 5'-R(*CP*CP*AP*GP*AP*(OMC)P*UP*(OMG)P*AP*AP*GP*AP*UP*(5MC)P*UP*GP*G)-3'

CCAGAXUXAA GAUXUGG

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5.60

#	Name	Isotope labeling	Type	Concentration
1	5'-R(*CP*CP*AP*GP*AP*(OMC)P*UP*(OMG)P*AP*AP*GP*AP*UP*(5MC)P*UP*GP*G)-3'			1.6 mM
2	cacodylate buffer			10 mM
3	EDTA			0.1 mM

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Release date

2005-11-13

Citation

Naturally-occurring modification restricts the anticodon domain conformational space of tRNA(Phe)
Stuart, J.W., Koshlap, K.M., Guenther, R.H., Agris, P.F.
J. Mol. Biol. (2003), 334, 901-918, PubMed 14643656 , DOI: , Abstract

Related entities 1. 5'-R(*CP*CP*AP*GP*AP*(OMC)P*UP*(OMG)P*AP*AP*GP*AP*UP*(5MC)P*UP*GP*G)-3', : 1 : 1 entities Detail

Experiments performed 2 experiments Detail