BMRB: 5532

Structure of the Tsg101 UEV Domain in Complex with a HIV-1 PTAP "Late Domain" Peptide

Authors

Pornillos, O., Alam, S.L., Davis, D.R., Sundquist, W.I.

Assembly

Tumor Susceptibility gene 101 protein/Gag Polyprotein

Entity

1. Tumor Susceptibility gene 101 protein (polymer, Thiol state: all free), 145 monomers, 16614.18 Da Detail

MAVSESQLKK MVSKYKYRDL TVRETVNVIT LYKDLKPVLD SYVFNDGSSR ELMNLTGTIP VPYRGNTYNI PICLWLLDTY PYNPPICFVK PTSSMTIKTG KHVDANGKIY LPYLHEWKHP QSDLLGLIQV MIVVFGDEPP VFSRP

2. Gag (polymer, Thiol state: not present), 9 monomers, 965.9968 Da Detail

PEPTAPPEE

Total weight

17580.176 Da

Max. entity weight

16614.18 Da

Exptl. method

NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 94.2 %, Completeness: 85.7 %, Completeness (bb): 90.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.7 % (1593 of 1858)	86.3 % (836 of 969)	83.1 % (615 of 740)	95.3 % (142 of 149)
Backbone	90.6 % (806 of 890)	94.0 % (280 of 298)	87.0 % (396 of 455)	94.9 % (130 of 137)
Sidechain	82.4 % (919 of 1115)	82.9 % (556 of 671)	81.3 % (351 of 432)	100.0 % (12 of 12)
Aromatic	59.6 % (93 of 156)	64.1 % (50 of 78)	53.9 % (41 of 76)	100.0 % (2 of 2)
Methyl	97.8 % (176 of 180)	97.8 % (88 of 90)	97.8 % (88 of 90)

1. Tumor Susceptibility gene 101 protein

MAVSESQLKK MVSKYKYRDL TVRETVNVIT LYKDLKPVLD SYVFNDGSSR ELMNLTGTIP VPYRGNTYNI PICLWLLDTY PYNPPICFVK PTSSMTIKTG KHVDANGKIY LPYLHEWKHP QSDLLGLIQV MIVVFGDEPP VFSRP

2. Gag

PEPTAPPEE

Show sample condition

Sample

Temperature 291 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	Tumor Susceptibility gene 101 protein			1.5 mM
2	Gag			1.5 mM

Hide sample codition

Chem. Shift Complete2

Sequence coverage: 27.9 %, Completeness: 52.0 %, Completeness (bb): 56.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	52.0 % (1933 of 3716)	51.8 % (1004 of 1938)	50.7 % (751 of 1480)	59.7 % (178 of 298)
Backbone	56.6 % (1007 of 1780)	57.9 % (345 of 596)	54.5 % (496 of 910)	60.6 % (166 of 274)
Sidechain	48.8 % (1089 of 2230)	49.1 % (659 of 1342)	48.4 % (418 of 864)	50.0 % (12 of 24)
Aromatic	36.5 % (114 of 312)	41.0 % (64 of 156)	31.6 % (48 of 152)	50.0 % (2 of 4)
Methyl	54.7 % (197 of 360)	55.0 % (99 of 180)	54.4 % (98 of 180)

1. Tumor Susceptibility gene 101 protein

MAVSESQLKK MVSKYKYRDL TVRETVNVIT LYKDLKPVLD SYVFNDGSSR ELMNLTGTIP VPYRGNTYNI PICLWLLDTY PYNPPICFVK PTSSMTIKTG KHVDANGKIY LPYLHEWKHP QSDLLGLIQV MIVVFGDEPP VFSRP

2. Gag

PEPTAPPEE

Show sample condition

Sample

Temperature 291 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	Tumor Susceptibility gene 101 protein			1.5 mM
2	Gag			1.5 mM

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 1M4P, Strand ID: A, B Detail

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Release date

2003-02-20

Citation

Structure of the Tsg101 UEV domain in complex with the PTAP motif of the HIV-1 p6 protein
Pornillos, O., Alam, S.L., Davis, D.R., Sundquist, W.I.
Nat. Struct. Biol. (2002), 9, 812-817, PubMed 12379843 , DOI 10.1038/nsb856 , Abstract

Related entities 1. Tumor Susceptibility gene 101 protein, : 1 : 21 entities Detail

Experiments performed 11 experiments Detail