BMRBj - BMREntryMaster

Found 1 Document (1.609 seconds)

BMRB: 5545

1RMJ

NMR assignment of the C-terminal domain of insulin-like growth factor binding protein-6 (IGFBP-6)

Authors

Headey, S.J., Yao, S., Parker, N.J., Kantharidis, P., Bach, L.A., Norton, R.S.

Assembly

insulin-like growth factor binding protein-6

Entity

1. insulin-like growth factor binding protein-6 (polymer, Thiol state: all disulfide bound), 107 monomers, 12056.15 Da Detail

SYYHHHHHHD YDIPTTENLY FQGAMGSGPC RRHLDSVLQQ LQTEVYRGAQ TLYVPNCDHR GFYRKRQCRS SQGQRRGPCW CVDRMGKSLP GSPDGNGSSS CPTGSSG

Formula weight

12056.15 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS30:SG	1:CYS57:SG
2	disulfide	sing	1:CYS68:SG	1:CYS79:SG
3	disulfide	sing	1:CYS81:SG	1:CYS101:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.5 %, Completeness: 83.9 %, Completeness (bb): 92.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.9 % (1004 of 1196)	85.5 % (538 of 629)	80.7 % (367 of 455)	88.4 % (99 of 112)
Backbone	92.0 % (578 of 628)	93.2 % (205 of 220)	90.9 % (280 of 308)	93.0 % (93 of 100)
Sidechain	77.5 % (513 of 662)	81.4 % (333 of 409)	72.2 % (174 of 241)	50.0 % (6 of 12)
Aromatic	31.7 % (38 of 120)	41.7 % (25 of 60)	20.3 % (12 of 59)	100.0 % (1 of 1)
Methyl	91.4 % (53 of 58)	89.7 % (26 of 29)	93.1 % (27 of 29)

1. insulin-like growth factor binding protein-6

SYYHHHHHHD YDIPTTENLY FQGAMGSGPC RRHLDSVLQQ LQTEVYRGAQ TLYVPNCDHR GFYRKRQCRS SQGQRRGPCW CVDRMGKSLP GSPDGNGSSS CPTGSSG

Show sample condition

Sample #1

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	insulin-like growth factor binding protein-6	[U-50% 15N]	1.0 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
5	insulin-like growth factor binding protein-6	[U-95% 13C; U-50% 15N]	1.0 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl protons	null	indirect	0.2514495
¹H	TMS	methyl protons	internal	direct	1.0
¹⁵N	TMS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl protons	null	indirect	0.2514495
¹H	TMS	methyl protons	internal	direct	1.0
¹⁵N	TMS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl protons	null	indirect	0.2514495
¹H	TMS	methyl protons	internal	direct	1.0
¹⁵N	TMS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl protons	null	indirect	0.2514495
¹H	TMS	methyl protons	internal	direct	1.0
¹⁵N	TMS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1RMJ, Strand ID: A Detail

Release date

2002-10-01

Citation

1H, 13C and 15N resonance assignments of the C-terminal domain of insulin-like growth factor binding protein-6 (IGFBP-6)
Headey, S.J., Yao, S., Parker, N.J., Kantharidis, P., Bach, L.A., Norton, R.S.
J. Biomol. NMR (2003), 25, 251-252, PubMed 12652138 , Abstract

Related entities 1. insulin-like growth factor binding protein-6, : 1 : 13 entities Detail

Experiments performed 8 experiments Detail

1 1H-15N NOESY-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	insulin-like growth factor binding protein-6	[U-50% 15N]	1.0 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
5	insulin-like growth factor binding protein-6	[U-95% 13C; U-50% 15N]	1.0 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

2 HNCA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	insulin-like growth factor binding protein-6	[U-50% 15N]	1.0 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
5	insulin-like growth factor binding protein-6	[U-95% 13C; U-50% 15N]	1.0 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

3 HNCACB

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	insulin-like growth factor binding protein-6	[U-50% 15N]	1.0 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
5	insulin-like growth factor binding protein-6	[U-95% 13C; U-50% 15N]	1.0 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

4 HNCO

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	insulin-like growth factor binding protein-6	[U-50% 15N]	1.0 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
5	insulin-like growth factor binding protein-6	[U-95% 13C; U-50% 15N]	1.0 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

5 HN(CA)CO

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	insulin-like growth factor binding protein-6	[U-50% 15N]	1.0 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
5	insulin-like growth factor binding protein-6	[U-95% 13C; U-50% 15N]	1.0 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

6 HNHA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	insulin-like growth factor binding protein-6	[U-50% 15N]	1.0 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
5	insulin-like growth factor binding protein-6	[U-95% 13C; U-50% 15N]	1.0 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

7 HAHB(CO)NH

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	insulin-like growth factor binding protein-6	[U-50% 15N]	1.0 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
5	insulin-like growth factor binding protein-6	[U-95% 13C; U-50% 15N]	1.0 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

8 1H-13C NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	insulin-like growth factor binding protein-6	[U-50% 15N]	1.0 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
5	insulin-like growth factor binding protein-6	[U-95% 13C; U-50% 15N]	1.0 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

nullKeywords IGFBP-6, NMR assignment, insulin-like growth factor

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Found 1 Document (1.609 seconds)

BMRB: 5545

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Chemical shift reference

Related entities 1. insulin-like growth factor binding protein-6, : 1 : 13 entities Detail

Experiments performed 8 experiments Detail

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