BMRB: 5552

Backbone 1H, 13C, and 15N Chemical Shift Assignments of the Catalytic Domain of Pac1

Authors

Farooq, A., Zhou, M.

Assembly

DUAL SPECIFICITY PROTEIN PHOSPHATASE 2 (E.C.3.1.3.48)(E.C.3.1.3.16)

Entity

1. DUAL SPECIFICITY PROTEIN PHOSPHATASE 2 (E.C.3.1.3.48)(E.C.3.1.3.16) (polymer, Thiol state: all free), 145 monomers, 16114.28 Da Detail

QGGPVEILPY LFLGSCSHSS DLQGLQACGI TAVLNVSASC PNHFEGLFRY KSIPVEDNQM VEISAWFQEA IGFIDWVKNS GGRVLVHSQA GISRSATICL AYLMQSRRVR LDEAFDFVKQ RRGVISPNFS FMGQLLQFET QVLCH

Formula weight

16114.28 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.8 %, Completeness: 61.1 %, Completeness (bb): 75.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	61.1 % (1032 of 1688)	63.1 % (550 of 872)	54.0 % (355 of 658)	80.4 % (127 of 158)
Backbone	75.8 % (652 of 860)	86.5 % (257 of 297)	64.1 % (271 of 423)	88.6 % (124 of 140)
Sidechain	52.3 % (503 of 961)	51.0 % (293 of 575)	56.3 % (207 of 368)	16.7 % (3 of 18)
Aromatic	20.1 % (33 of 164)	31.7 % (26 of 82)	6.3 % (5 of 80)	100.0 % (2 of 2)
Methyl	75.6 % (124 of 164)	78.0 % (64 of 82)	73.2 % (60 of 82)

1. dual specificity protein phosphatase 2

QGGPVEILPY LFLGSCSHSS DLQGLQACGI TAVLNVSASC PNHFEGLFRY KSIPVEDNQM VEISAWFQEA IGFIDWVKNS GGRVLVHSQA GISRSATICL AYLMQSRRVR LDEAFDFVKQ RRGVISPNFS FMGQLLQFET QVLCH

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	dual specificity protein phosphatase 2	[U-13C; U-15N]		0.5 mM
2	phosphate buffer			5 mM

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Calculated from 1 models in PDB: 1M3G, Strand ID: A Detail

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Release date

2003-03-13

Citation

Solution structure of the MAPK phosphatase PAC-1 catalytic domain. Insights into substrate-induced enzymatic activation of MKP
Farooq, A., Plotnikova, O., Chaturvedi, G., Yan, S., Zeng, L., Zhang, Q., Zhou, M.
Structure (2003), 11, 155-164, PubMed 12575935 , DOI: , Abstract

Related entities 1. DUAL SPECIFICITY PROTEIN PHOSPHATASE 2 (E.C.3.1.3.48)(E.C.3.1.3.16), : 1 : 2 : 273 entities Detail

Interaction partners 1. DUAL SPECIFICITY PROTEIN PHOSPHATASE 2 (E.C.3.1.3.48)(E.C.3.1.3.16), : 1 interactors Detail

Experiments performed 3 experiments Detail