BMRB: 5554

Structure of the N-WASP EVH1 Domain-WIP complex

Authors

Volkman, B.F., Prehoda, K.E., Scott, J.A., Peterson, F.C., Lim, W.A.

Assembly

N-WASP EVH1 Domain-WIP complex

Entity

1. N-WASP EVH1 Domain-WIP complex (polymer, Thiol state: all free), 144 monomers, 16339.42 Da Detail

DLPPPEPYVQ TTKSYPSKLA RNESRGSGSG SLFSFLGKKC VTMSSAVVQL YAADRNCMWS KKCSGVACLV KDNPQRSYFL RIFDIKDGKL LWEQELYNNF VYNSPRGYFH TFAGDTCQVA LNFANEEEAK KFRKAVTDLL GRRQ

Formula weight

16339.42 Da

Source organism

Rattus rattus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.6 %, Completeness: 81.0 %, Completeness (bb): 83.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	81.0 % (1386 of 1711)	81.8 % (733 of 896)	80.2 % (531 of 662)	79.7 % (122 of 153)
Backbone	83.9 % (713 of 850)	84.8 % (246 of 290)	83.9 % (355 of 423)	81.8 % (112 of 137)
Sidechain	78.1 % (778 of 996)	79.4 % (481 of 606)	77.0 % (288 of 374)	56.3 % (9 of 16)
Aromatic	69.0 % (120 of 174)	85.1 % (74 of 87)	51.8 % (44 of 85)	100.0 % (2 of 2)
Methyl	91.4 % (117 of 128)	92.2 % (59 of 64)	90.6 % (58 of 64)

1. WIP PEPTIDE AND N-WASP EVH1 DOMAIN

DLPPPEPYVQ TTKSYPSKLA RNESRGSGSG SLFSFLGKKC VTMSSAVVQL YAADRNCMWS KKCSGVACLV KDNPQRSYFL RIFDIKDGKL LWEQELYNNF VYNSPRGYFH TFAGDTCQVA LNFANEEEAK KFRKAVTDLL GRRQ

Show sample condition

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	WIP PEPTIDE AND N-WASP EVH1 DOMAIN	[U-15N]		1 mM
2	phosphate buffer			20 mM
3	dithiothreitol			1 mM
4	H2O			90 %
5	D2O			10 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	WIP PEPTIDE AND N-WASP EVH1 DOMAIN	[U-15N; U-13C]		1 mM
7	phosphate buffer			20 mM
8	dithiothreitol			1 mM
9	H2O			90 %
10	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.25 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.25 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.17 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 21 models in PDB: 1MKE, Strand ID: A Detail

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Release date

2003-02-20

Citation

Structure of the N-WASP EVH1 domain-WIP complex: insight into the molecular basis of Wiskott-Aldrich Syndrome
Volkman, B.F., Prehoda, K.E., Scott, J.A., Peterson, F.C., Lim, W.A.
Cell (2002), 111, 565-576, PubMed 12437929 , DOI: , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. N-WASP EVH1 Domain-WIP complex, : 1 : 5 entities Detail

Interaction partners 1. N-WASP EVH1 Domain-WIP complex, : 45 interactors Detail

Experiments performed 2 experiments Detail