BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	68.3 % (884 of 1294)	65.9 % (455 of 690)	66.6 % (331 of 497)	91.6 % (98 of 107)
Backbone	77.1 % (472 of 612)	91.4 % (191 of 209)	63.0 % (192 of 305)	90.8 % (89 of 98)
Sidechain	63.9 % (498 of 779)	54.9 % (264 of 481)	77.9 % (225 of 289)	100.0 % (9 of 9)
Aromatic	50.0 % (57 of 114)	49.1 % (28 of 57)	49.1 % (27 of 55)	100.0 % (2 of 2)
Methyl	84.2 % (64 of 76)	78.9 % (30 of 38)	89.5 % (34 of 38)

1. SAND domain from human Sp100b protein

MKHHHHHHPM DENINFKQSE LPVTCGEVKG TLYKERFKQG TSKKCIQSED KKWFTPREFE IEGDRGASKN WKLSIRCGGY TLKVLMENKF LPEPPSTRKK VTIK

Show sample condition

Sample #1

Temperature 295 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	SAND domain from human Sp100b protein	[U-95% 15N]	1.2 mM
2	sodium phosphate		20 mM
3	sodium chloride		50 mM
4	DTT		4 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	SAND domain from human Sp100b protein	[U-95% 13C; U-15N]	1.2 mM
6	sodium phosphate		20 mM
7	sodium chloride		50 mM
8	DTT		4 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 2.47 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 2.47 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.02 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1H5P, Strand ID: A Detail

Coupling constant

30 J values in 1 lists
Temperature 295 (±0.5) K, pH 6.5 (±0.2) Detail

Release date

2002-12-26

Citation

The SAND domain structure defines a novel DNA-binding fold in transcriptional regulation
Bottomley, M.J., Collard, M.W., Huggenvik, J.I., Liu, Z., Gibson, T.J., Sattler, M.
Nat. Struct. Biol. (2001), 8, 626-633, PubMed 11427895 , DOI 10.1038/89675 , Abstract

Related entities 1. Sp100b SAND domain, : 1 : 25 entities Detail

Interaction partners 1. Sp100b SAND domain, : 36 interactors Detail

More details for 36 interactors identified by 18 citations

Experiments performed 7 experiments Detail

1 HNCA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (700 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	SAND domain from human Sp100b protein	[U-95% 15N]	1.2 mM
2	sodium phosphate		20 mM
3	sodium chloride		50 mM
4	DTT		4 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	SAND domain from human Sp100b protein	[U-95% 13C; U-15N]	1.2 mM
6	sodium phosphate		20 mM
7	sodium chloride		50 mM
8	DTT		4 mM

2 CBCANH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (700 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	SAND domain from human Sp100b protein	[U-95% 15N]	1.2 mM
2	sodium phosphate		20 mM
3	sodium chloride		50 mM
4	DTT		4 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	SAND domain from human Sp100b protein	[U-95% 13C; U-15N]	1.2 mM
6	sodium phosphate		20 mM
7	sodium chloride		50 mM
8	DTT		4 mM

3 CBCA(CO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (700 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	SAND domain from human Sp100b protein	[U-95% 15N]	1.2 mM
2	sodium phosphate		20 mM
3	sodium chloride		50 mM
4	DTT		4 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	SAND domain from human Sp100b protein	[U-95% 13C; U-15N]	1.2 mM
6	sodium phosphate		20 mM
7	sodium chloride		50 mM
8	DTT		4 mM

4 H(C)CH TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (700 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	SAND domain from human Sp100b protein	[U-95% 15N]	1.2 mM
2	sodium phosphate		20 mM
3	sodium chloride		50 mM
4	DTT		4 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	SAND domain from human Sp100b protein	[U-95% 13C; U-15N]	1.2 mM
6	sodium phosphate		20 mM
7	sodium chloride		50 mM
8	DTT		4 mM

5 CCH TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (700 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	SAND domain from human Sp100b protein	[U-95% 15N]	1.2 mM
2	sodium phosphate		20 mM
3	sodium chloride		50 mM
4	DTT		4 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	SAND domain from human Sp100b protein	[U-95% 13C; U-15N]	1.2 mM
6	sodium phosphate		20 mM
7	sodium chloride		50 mM
8	DTT		4 mM

6 15N-edited NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (700 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	SAND domain from human Sp100b protein	[U-95% 15N]	1.2 mM
2	sodium phosphate		20 mM
3	sodium chloride		50 mM
4	DTT		4 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	SAND domain from human Sp100b protein	[U-95% 13C; U-15N]	1.2 mM
6	sodium phosphate		20 mM
7	sodium chloride		50 mM
8	DTT		4 mM

7 13C-edited NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (700 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	SAND domain from human Sp100b protein	[U-95% 15N]	1.2 mM
2	sodium phosphate		20 mM
3	sodium chloride		50 mM
4	DTT		4 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	SAND domain from human Sp100b protein	[U-95% 13C; U-15N]	1.2 mM
6	sodium phosphate		20 mM
7	sodium chloride		50 mM
8	DTT		4 mM

nullKeywords SAND domain, Sp100b, KDWK

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Found 1 Document (1.913 seconds)

BMRB: 5558

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Sp100b SAND domain, : 1 : 25 entities Detail

Interaction partners 1. Sp100b SAND domain, : 36 interactors Detail

Experiments performed 7 experiments Detail

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