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Found 1 Document (1.685 seconds)

BMRB: 5560

1RDU

NMR assignment of the conserved hypothetical protein TM1290 of Thermotoga maritima

Authors

Etezady-Esfarjani, T., Peti, W., Wuethrich, K.

Assembly

TM1290 conserved hypothetical protein

Entity

1. TM1290 conserved hypothetical protein (polymer, Thiol state: not present), 116 monomers, 12537.90 Da Detail

MARVAIPSVG KDLSSMVSDR FARAEYFIIY DTESGNVEVV ENTIADAHGT GPKVVQSLVS KGVEYLIASN VGRNAFETLK AAGVKVYRFE GGTVQEAIDA FSEGRLEELT TFTREG

Formula weight

12537.9 Da

Source organism

Thermotoga maritima

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 89.6 %, Completeness (bb): 92.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.6 % (1151 of 1284)	92.4 % (606 of 656)	86.8 % (441 of 508)	86.7 % (104 of 120)
Backbone	92.8 % (642 of 692)	92.5 % (223 of 241)	93.5 % (315 of 337)	91.2 % (104 of 114)
Sidechain	87.5 % (610 of 697)	92.3 % (383 of 415)	82.2 % (227 of 276)	0.0 % (0 of 6)
Aromatic	56.3 % (54 of 96)	97.9 % (47 of 48)	14.6 % (7 of 48)
Methyl	98.6 % (142 of 144)	98.6 % (71 of 72)	98.6 % (71 of 72)

1. Tm1290

MARVAIPSVG KDLSSMVSDR FARAEYFIIY DTESGNVEVV ENTIADAHGT GPKVVQSLVS KGVEYLIASN VGRNAFETLK AAGVKVYRFE GGTVQEAIDA FSEGRLEELT TFTREG

Show sample condition

Sample #1

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Tm1290	[U-98% 13C; U-98% 15N]		3.8 mM

Sample #2

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Tm1290	[U-98% 15N]		2.3 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.14 ppm, Outliers: 6 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.14 ppm, Outliers: 6 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.04 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.38 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1RDU, Strand ID: A Detail

Release date

2003-02-17

Citation

NMR assignment of the conserved hypothetical protein TM1290 of Thermotoga maritima
Etezady-Esfarjani, T., Peti, W., Wuethrich, K.
J. Biomol. NMR (2003), 25, 167-168, PubMed 12652129 , DOI: , Abstract

Related entities 1. TM1290 conserved hypothetical protein, : 1 : 13 entities Detail

Experiments performed 9 experiments Detail

1 1H-15N TOCSY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Tm1290	[U-98% 13C; U-98% 15N]		3.8 mM

Sample #2

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Tm1290	[U-98% 15N]		2.3 mM

2 HNCACB

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Tm1290	[U-98% 13C; U-98% 15N]		3.8 mM

Sample #2

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Tm1290	[U-98% 15N]		2.3 mM

3 HNCO

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Tm1290	[U-98% 13C; U-98% 15N]		3.8 mM

Sample #2

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Tm1290	[U-98% 15N]		2.3 mM

4 CBCA(CO)NH

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Tm1290	[U-98% 13C; U-98% 15N]		3.8 mM

Sample #2

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Tm1290	[U-98% 15N]		2.3 mM

5 1H-13C NOESY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Tm1290	[U-98% 13C; U-98% 15N]		3.8 mM

Sample #2

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Tm1290	[U-98% 15N]		2.3 mM

6 1H-1H-15N-resolved NOESY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Tm1290	[U-98% 13C; U-98% 15N]		3.8 mM

Sample #2

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Tm1290	[U-98% 15N]		2.3 mM

7 1H-1H NOESY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Tm1290	[U-98% 13C; U-98% 15N]		3.8 mM

Sample #2

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Tm1290	[U-98% 15N]		2.3 mM

8 1H-15N HSQC

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Tm1290	[U-98% 13C; U-98% 15N]		3.8 mM

Sample #2

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Tm1290	[U-98% 15N]		2.3 mM

9 1H-13C HSQC

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Tm1290	[U-98% 13C; U-98% 15N]		3.8 mM

Sample #2

Temperature 313 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Tm1290	[U-98% 15N]		2.3 mM

nullKeywords hypothetical protein, NMR assignment, structural genomic, Thermotoga Maritima, triple resonance experiments

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Found 1 Document (1.685 seconds)

BMRB: 5560

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. TM1290 conserved hypothetical protein, : 1 : 13 entities Detail

Experiments performed 9 experiments Detail

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