BMRBj - BMREntryMaster

Found 1 Document (1.622 seconds)

BMRB: 5561

1O6X

NMR solution structure of the activation domain of human procarboxipeptidase A2

Authors

Jimenez, M., Villegas, V., Santoro, J., Serrano, L., Vendrell, J., Aviles, X., Rico, M.

Assembly

Activation domain of human procarboxipeptidase A2

Entity

1. Activation domain of human procarboxipeptidase A2 (polymer, Thiol state: not present), 81 monomers, 9210.360 Da Detail

MRSLETFVGD QVLEIVPSNE EQIKNLLQLE AQEHLQLDFW KSPTTPGETA HVRVPFVNVQ AVKVFLESQG IAYSIMIEDV Q

Formula weight

9210.36 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.0 %, Completeness (bb): 96.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	97.0 % (573 of 591)	97.2 % (488 of 502)	95.5 % (85 of 89)
Backbone	96.2 % (229 of 238)	96.9 % (156 of 161)	94.8 % (73 of 77)
Sidechain	97.2 % (343 of 353)	97.1 % (331 of 341)	100.0 % (12 of 12)
Aromatic	94.3 % (33 of 35)	94.1 % (32 of 34)	100.0 % (1 of 1)
Methyl	98.1 % (53 of 54)	98.1 % (53 of 54)

1. Activation domain of human procarboxipeptidase

MRSLETFVGD QVLEIVPSNE EQIKNLLQLE AQEHLQLDFW KSPTTPGETA HVRVPFVNVQ AVKVFLESQG IAYSIMIEDV Q

Show sample condition

Sample #1

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Activation domain of human procarboxipeptidase			2.0 mM
2	sodium phosphate buffer			50 mM

Sample #2

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Activation domain of human procarboxipeptidase	[U-95.5% 15N]		2.0 mM
4	sodium phosphate buffer			50 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 1O6X, Strand ID: A Detail

Release date

2003-02-20

Citation 1

NMR solution structure of the activation domain of human procarboxypeptidase A2
Jimenez, M., Villegas, V., Santoro, J., Serrano, L., Vendrell, J., Aviles, F., Rico, M.
Protein Sci. (2003), 12, 296-305, PubMed 12538893 , DOI 10.1110/ps.0227303 , Abstract

Show more 1 citaion

Citation 2

The sequence and conformation of human pancreatic procarboxypeptidase A2. cDNA cloning, sequence analysis, and three-dimensional model
Catasus, L., Vendrell, J., Aviles, F.X., Carreira, S., Puigserver, A., Billeter, M.
J. Biol. Chem. (1995), 270, 6651-6657, PubMed 7896805 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. Activation domain of human procarboxipeptidase A2, : 1 : 3 : 55 entities Detail

Interaction partners 1. Activation domain of human procarboxipeptidase A2, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 7 experiments Detail

1 2D 1H-1H COSY

Instrument

Model Bruker AMX (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Activation domain of human procarboxipeptidase			2.0 mM
2	sodium phosphate buffer			50 mM

Sample #2

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Activation domain of human procarboxipeptidase	[U-95.5% 15N]		2.0 mM
4	sodium phosphate buffer			50 mM

2 2D 1H-1H TOCSY

Instrument

Model Bruker AMX (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Activation domain of human procarboxipeptidase			2.0 mM
2	sodium phosphate buffer			50 mM

Sample #2

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Activation domain of human procarboxipeptidase	[U-95.5% 15N]		2.0 mM
4	sodium phosphate buffer			50 mM

3 2D 1H-1H NOESY

Instrument

Model Bruker AMX (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Activation domain of human procarboxipeptidase			2.0 mM
2	sodium phosphate buffer			50 mM

Sample #2

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Activation domain of human procarboxipeptidase	[U-95.5% 15N]		2.0 mM
4	sodium phosphate buffer			50 mM

4 2D 1H-15N HSQC

Instrument

Model Bruker AMX (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Activation domain of human procarboxipeptidase			2.0 mM
2	sodium phosphate buffer			50 mM

Sample #2

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Activation domain of human procarboxipeptidase	[U-95.5% 15N]		2.0 mM
4	sodium phosphate buffer			50 mM

5 3D 1H-1H-15N NOESY

Instrument

Model Bruker AMX (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Activation domain of human procarboxipeptidase			2.0 mM
2	sodium phosphate buffer			50 mM

Sample #2

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Activation domain of human procarboxipeptidase	[U-95.5% 15N]		2.0 mM
4	sodium phosphate buffer			50 mM

6 3D 1H-1H-15N TOCSY

Instrument

Model Bruker AMX (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Activation domain of human procarboxipeptidase			2.0 mM
2	sodium phosphate buffer			50 mM

Sample #2

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Activation domain of human procarboxipeptidase	[U-95.5% 15N]		2.0 mM
4	sodium phosphate buffer			50 mM

7 2D 1H-13C HSQC

Instrument

Model Bruker AMX (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Activation domain of human procarboxipeptidase			2.0 mM
2	sodium phosphate buffer			50 mM

Sample #2

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Activation domain of human procarboxipeptidase	[U-95.5% 15N]		2.0 mM
4	sodium phosphate buffer			50 mM

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Found 1 Document (1.622 seconds)

BMRB: 5561

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Related entities 1. Activation domain of human procarboxipeptidase A2, : 1 : 3 : 55 entities Detail

Interaction partners 1. Activation domain of human procarboxipeptidase A2, : 1 interactors Detail

Experiments performed 7 experiments Detail

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