BMRB: 5567

1H, 15N and 13C Resonance Assignments for a Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1

Authors

Manzur, K., Farooq, A., Zeng, L., Zhou, M.

Assembly

Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1

Entity

1. Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1 (polymer, Thiol state: not reported), 119 monomers, 13586.46 × 2 Da Detail

MFNDRVIVKK SPLGGYGVFA RKSFEKGELV EECLCIVRHN DDWGTALEDY LFSRKNMSAM ALGFGAIFNH SKDPNARHEL TAGLKRMRIF TIKPIAIGEE ITISYGDDYW LSRPRLTQN

Total weight

27172.92 Da

Max. entity weight

13586.46 Da

Source organism

Paramecium bursaria

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 74.2 %, Completeness (bb): 82.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	74.2 % (1050 of 1415)	76.0 % (561 of 738)	68.2 % (377 of 553)	90.3 % (112 of 124)
Backbone	82.2 % (580 of 706)	93.0 % (227 of 244)	70.0 % (243 of 347)	95.7 % (110 of 115)
Sidechain	70.3 % (575 of 818)	67.6 % (334 of 494)	75.9 % (239 of 315)	22.2 % (2 of 9)
Aromatic	43.5 % (60 of 138)	47.8 % (33 of 69)	40.3 % (27 of 67)	0.0 % (0 of 2)
Methyl	96.7 % (118 of 122)	96.7 % (59 of 61)	96.7 % (59 of 61)

1. Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1

MFNDRVIVKK SPLGGYGVFA RKSFEKGELV EECLCIVRHN DDWGTALEDY LFSRKNMSAM ALGFGAIFNH SKDPNARHEL TAGLKRMRIF TIKPIAIGEE ITISYGDDYW LSRPRLTQN

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Sample

Temperature 310 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1			0.5 mM

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LACS Plot; CA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl proton	0.0 ppm	external	indirect	1.0

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LACS Plot; CB

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl proton	0.0 ppm	external	indirect	1.0

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl proton	0.0 ppm	external	indirect	1.0

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Protein Blocks Logo

Calculated from 1 models in PDB: 1N3J, Strand ID: A, B Detail

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Release date

2003-06-09

Citation

1H, 13C and 15N resonance assignments of a viral SET domain histone lysine methyltransferase
Manzur, K., Farooq, A., Zeng, L., Zhou, M.
J. Biomol. NMR (2003), 26, 279-280, PubMed 12766424 , DOI: , Abstract

Related entities 1. Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1, : 1 : 4 : 109 entities Detail