BMRB: 5576

Sodium/potassium-transporting ATPase alpha-1 chain in complex with ATP

Authors

Hilge, M., Siegal, G., Vuister, G.W., Guentert, P., Gloor, S.M., Abrahams, J.P.

Assembly

nbd-min001

Entity

1. SODIUM/POTASSIUM-TRANSPORTING ATPASE ALPHA-1 (polymer, Thiol state: all free), 213 monomers, 23844.89 Da Detail

QNPMTVAHMW FDNQIHEADT TENQSGVSFD KTSATWFALS RIAGLCNRAV FQANQENLPI LKRAVAGDAS ESALLKCIEV CCGSVMEMRE KYTKIVEIPF NSTNKYQLSI HKNPNASEPK HLLVMKGAPE RILDRCSSIL LHGKEQPLDE ELKDAFQNAY LELGGLGERV LGFCHLLLPD EQFPEGFQFD TDEVNFPVDN LCFVGLISMI DPP

2. ATP (non-polymer), 507.181 Da

Total weight

24352.072 Da

Max. entity weight

23844.89 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

Automated NOESY cross peak assignment

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 96.7 %, Completeness: 82.9 %, Completeness (bb): 81.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.9 % (2060 of 2484)	88.2 % (1140 of 1292)	74.5 % (720 of 966)	88.5 % (200 of 226)
Backbone	81.0 % (1016 of 1254)	93.0 % (396 of 426)	69.4 % (435 of 627)	92.0 % (185 of 201)
Sidechain	86.1 % (1232 of 1431)	85.9 % (744 of 866)	87.4 % (472 of 540)	64.0 % (16 of 25)
Aromatic	63.5 % (122 of 192)	67.7 % (65 of 96)	58.5 % (55 of 94)	100.0 % (2 of 2)
Methyl	98.3 % (226 of 230)	98.3 % (113 of 115)	98.3 % (113 of 115)

1. SODIUM/POTASSIUM-TRANSPORTING ATPASE ALPHA-1

QNPMTVAHMW FDNQIHEADT TENQSGVSFD KTSATWFALS RIAGLCNRAV FQANQENLPI LKRAVAGDAS ESALLKCIEV CCGSVMEMRE KYTKIVEIPF NSTNKYQLSI HKNPNASEPK HLLVMKGAPE RILDRCSSIL LHGKEQPLDE ELKDAFQNAY LELGGLGERV LGFCHLLLPD EQFPEGFQFD TDEVNFPVDN LCFVGLISMI DPP

Show sample condition

Sample

Pressure 1 atm, Temperature 296 K, pH 8.6

#	Name	Isotope labeling	Type	Concentration
1	SODIUM/POTASSIUM-TRANSPORTING ATPASE ALPHA-1	[U-13C; U-15N]		2 mM
2	ADENOSINE-5'-TRIPHOSPHATE			0.0 ~ 0.0 mM
3	Tris-HCl			20 mM
4	NaN3			0.02 %

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Chem. Shift Complete2

Sequence coverage: 26.8 %, Completeness: 50.2 %, Completeness (bb): 52.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	50.2 % (2494 of 4968)	51.9 % (1342 of 2584)	46.6 % (900 of 1932)	55.8 % (252 of 452)
Backbone	52.0 % (1303 of 2508)	57.2 % (487 of 852)	46.3 % (580 of 1254)	58.7 % (236 of 402)
Sidechain	49.9 % (1428 of 2862)	49.4 % (855 of 1732)	51.5 % (556 of 1080)	34.0 % (17 of 50)
Aromatic	35.7 % (137 of 384)	39.6 % (76 of 192)	31.4 % (59 of 188)	50.0 % (2 of 4)
Methyl	58.7 % (270 of 460)	58.7 % (135 of 230)	58.7 % (135 of 230)

1. SODIUM/POTASSIUM-TRANSPORTING ATPASE ALPHA-1

QNPMTVAHMW FDNQIHEADT TENQSGVSFD KTSATWFALS RIAGLCNRAV FQANQENLPI LKRAVAGDAS ESALLKCIEV CCGSVMEMRE KYTKIVEIPF NSTNKYQLSI HKNPNASEPK HLLVMKGAPE RILDRCSSIL LHGKEQPLDE ELKDAFQNAY LELGGLGERV LGFCHLLLPD EQFPEGFQFD TDEVNFPVDN LCFVGLISMI DPP

Show sample condition

Sample

Pressure 1 atm, Temperature 296 K, pH 8.6

#	Name	Isotope labeling	Type	Concentration
1	SODIUM/POTASSIUM-TRANSPORTING ATPASE ALPHA-1	[U-13C; U-15N]		2 mM
2	ADENOSINE-5'-TRIPHOSPHATE			0.0 ~ 0.0 mM
3	Tris-HCl			20 mM
4	NaN3			0.02 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 1MO8, Strand ID: A Detail

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Release date

2005-11-13

Citation

ATP-induced conformational changes of the nucleotide-binding domain of Na,K-ATPase
Hilge, M., Siegal, G., Vuister, G.W., Guntert, P., Gloor, S.M., Abrahams, J.P.
Nat. Struct. Biol. (2003), 10, 468-474, PubMed 12730684 , DOI 10.1038/nsb924 , Abstract

Related entities 1. SODIUM/POTASSIUM-TRANSPORTING ATPASE ALPHA-1, : 1 : 1 : 2 : 258 entities Detail

Interaction partners 1. SODIUM/POTASSIUM-TRANSPORTING ATPASE ALPHA-1, : 5 interactors Detail

Experiments performed 2 experiments Detail