BMRB: 5577

Sodium/potassium-transporting ATPase alpha-1 chain

Authors

Hilge, M., Siegal, G., Vuister, G.W., Guentert, P., Gloor, S.M., Abrahams, J.P.

Assembly

sodium/potassium-transporting ATPase alpha-1 chain (E.C.3.6.3.9)

Entity

1. sodium/potassium-transporting ATPase alpha-1 chain (E.C.3.6.3.9) (polymer, Thiol state: all free), 213 monomers, 23844.89 Da Detail

QNPMTVAHMW FDNQIHEADT TENQSGVSFD KTSATWFALS RIAGLCNRAV FQANQENLPI LKRAVAGDAS ESALLKCIEV CCGSVMEMRE KYTKIVEIPF NSTNKYQLSI HKNPNASEPK HLLVMKGAPE RILDRCSSIL LHGKEQPLDE ELKDAFQNAY LELGGLGERV LGFCHLLLPD EQFPEGFQFD TDEVNFPVDN LCFVGLISMI DPP

Formula weight

23844.89 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

automated NOESY cross peak assignment

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 81.7 %, Completeness (bb): 80.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	81.7 % (2030 of 2484)	86.0 % (1111 of 1292)	75.2 % (726 of 966)	85.4 % (193 of 226)
Backbone	80.4 % (1008 of 1254)	92.5 % (394 of 426)	69.2 % (434 of 627)	89.6 % (180 of 201)
Sidechain	84.6 % (1210 of 1431)	82.8 % (717 of 866)	88.9 % (480 of 540)	52.0 % (13 of 25)
Aromatic	64.1 % (123 of 192)	64.6 % (62 of 96)	62.8 % (59 of 94)	100.0 % (2 of 2)
Methyl	96.5 % (222 of 230)	94.8 % (109 of 115)	98.3 % (113 of 115)

1. SODIUM/POTASSIUM-TRANSPORTING ATPASE ALPHA-1

QNPMTVAHMW FDNQIHEADT TENQSGVSFD KTSATWFALS RIAGLCNRAV FQANQENLPI LKRAVAGDAS ESALLKCIEV CCGSVMEMRE KYTKIVEIPF NSTNKYQLSI HKNPNASEPK HLLVMKGAPE RILDRCSSIL LHGKEQPLDE ELKDAFQNAY LELGGLGERV LGFCHLLLPD EQFPEGFQFD TDEVNFPVDN LCFVGLISMI DPP

Show sample condition

Sample

Pressure 1 atm, Temperature 296 K, pH 8.6

#	Name	Isotope labeling	Type	Concentration
1	SODIUM/POTASSIUM-TRANSPORTING ATPASE ALPHA-1	[U-13C; U-15N]		2 mM
2	Tris-HCl			20 mM
3	NaN3			0.02 %
4	H2O			95 %
5	D2O			5 %

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LACS Plot; CA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 1MO7, Strand ID: A Detail

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Release date

2003-07-31

Citation

ATP-induced conformational changes of the nucleotide-binding domain of Na,K-ATPase
Hilge, M., Siegal, G., Vuister, G.W., Guntert, P., Gloor, S.M., Abrahams, J.P.
Nat. Struct. Biol. (2003), 10, 468-474, PubMed 12730684 , DOI 10.1038/nsb924 , Abstract

Related entities 1. sodium/potassium-transporting ATPase alpha-1 chain (E.C.3.6.3.9), : 1 : 1 : 2 : 258 entities Detail

Interaction partners 1. sodium/potassium-transporting ATPase alpha-1 chain (E.C.3.6.3.9), : 5 interactors Detail

Experiments performed 2 experiments Detail