BMRBj - BMREntryMaster

Found 1 Document (2.793 seconds)

BMRB: 5578

1MX8

Two Homologous Rat Cellular Retinol-binding Proteins Differ in Local Conformational Flexibility

Authors

Lu, J., Cistola, D.P., Li, E.

Assembly

rat cellular retinol-binding protein I, bound to all-trans retinol

Entity

1. rat cellular retinol-binding protein I (polymer, Thiol state: all free), 134 monomers, 15702.69 Da Detail

PVDFNGYWKM LSNENFEEYL RALDVNVALR KIANLLKPDK EIVQDGDHMI IRTLSTFRNY IMDFQVGKEF EEDLTGIDDR KCMTTVSWDG DKLQCVQKGE KEGRGWTQWI EGDELHLEMR AEGVTCKQVF KKVH

2. RETINOL (non-polymer), 1 monomers, 286.452 Da

Total weight

15989.143 Da

Max. entity weight

15702.69 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 93.1 %, Completeness (bb): 99.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.1 % (1509 of 1620)	96.0 % (814 of 848)	87.7 % (547 of 624)	100.0 % (148 of 148)
Backbone	99.6 % (797 of 800)	100.0 % (276 of 276)	99.2 % (389 of 392)	100.0 % (132 of 132)
Sidechain	88.5 % (835 of 944)	94.1 % (538 of 572)	78.9 % (281 of 356)	100.0 % (16 of 16)
Aromatic	40.3 % (58 of 144)	75.0 % (54 of 72)	0.0 % (0 of 68)	100.0 % (4 of 4)
Methyl	98.5 % (132 of 134)	97.0 % (65 of 67)	100.0 % (67 of 67)

1. rat cellular retinol-binding protein I

PVDFNGYWKM LSNENFEEYL RALDVNVALR KIANLLKPDK EIVQDGDHMI IRTLSTFRNY IMDFQVGKEF EEDLTGIDDR KCMTTVSWDG DKLQCVQKGE KEGRGWTQWI EGDELHLEMR AEGVTCKQVF KKVH

Show sample condition

Sample

Temperature 298 (±1) K, pH 7.4 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Chem. Shift Complete2

Sequence coverage: 20.9 %, Completeness: 52.4 %, Completeness (bb): 57.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	52.4 % (1699 of 3240)	53.4 % (906 of 1696)	49.8 % (621 of 1248)	58.1 % (172 of 296)
Backbone	57.4 % (919 of 1600)	57.1 % (315 of 552)	57.3 % (449 of 784)	58.7 % (155 of 264)
Sidechain	48.7 % (920 of 1888)	51.7 % (591 of 1144)	43.8 % (312 of 712)	53.1 % (17 of 32)
Aromatic	20.1 % (58 of 288)	37.5 % (54 of 144)	0.0 % (0 of 136)	50.0 % (4 of 8)
Methyl	53.0 % (142 of 268)	52.2 % (70 of 134)	53.7 % (72 of 134)

1. rat cellular retinol-binding protein I

PVDFNGYWKM LSNENFEEYL RALDVNVALR KIANLLKPDK EIVQDGDHMI IRTLSTFRNY IMDFQVGKEF EEDLTGIDDR KCMTTVSWDG DKLQCVQKGE KEGRGWTQWI EGDELHLEMR AEGVTCKQVF KKVH

Show sample condition

Sample

Temperature 298 (±1) K, pH 7.4 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Protein Blocks Logo

Calculated from 25 models in PDB: 1MX8, Strand ID: A Detail

Release date

2003-07-24

Citation

Two homologous rat cellular retinol-binding proteins differ in local conformational flexibility
Lu, J., Cistola, D.P., Li, E.
J. Mol. Biol. (2003), 330, 799-812, PubMed 12850148 , Abstract

Related entities 1. rat cellular retinol-binding protein I, : 1 : 6 : 24 : 213 entities Detail

Interaction partners 1. rat cellular retinol-binding protein I, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 8 experiments Detail

1 HNCO

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian UNITYPLUS (500 MHz)

#4 Model Varian UNITY (600 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	rat cellular retinol-binding protein I	[U-80% 2H; U-99% 15N]		1.0 mM

2 HNCACB

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian UNITYPLUS (500 MHz)

#4 Model Varian UNITY (600 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	rat cellular retinol-binding protein I	[U-80% 2H; U-99% 15N]		1.0 mM

3 CBCACONNH

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian UNITYPLUS (500 MHz)

#4 Model Varian UNITY (600 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	rat cellular retinol-binding protein I	[U-80% 2H; U-99% 15N]		1.0 mM

4 CBCACOCAHA

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian UNITYPLUS (500 MHz)

#4 Model Varian UNITY (600 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	rat cellular retinol-binding protein I	[U-80% 2H; U-99% 15N]		1.0 mM

5 1H-15N NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian UNITYPLUS (500 MHz)

#4 Model Varian UNITY (600 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	rat cellular retinol-binding protein I	[U-80% 2H; U-99% 15N]		1.0 mM

6 1H-15N TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian UNITYPLUS (500 MHz)

#4 Model Varian UNITY (600 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	rat cellular retinol-binding protein I	[U-80% 2H; U-99% 15N]		1.0 mM

7 HCCH-TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian UNITYPLUS (500 MHz)

#4 Model Varian UNITY (600 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	rat cellular retinol-binding protein I	[U-80% 2H; U-99% 15N]		1.0 mM

8 1H-13C NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian UNITYPLUS (500 MHz)

#4 Model Varian UNITY (600 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	rat cellular retinol-binding protein I	[U-80% 2H; U-99% 15N]		1.0 mM

nullKeywords cellular retinol-binding protein, vitamin A, NMR, structure, dynamics

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Found 1 Document (2.793 seconds)

BMRB: 5578

Sample

Sample

Related entities 1. rat cellular retinol-binding protein I, : 1 : 6 : 24 : 213 entities Detail

Interaction partners 1. rat cellular retinol-binding protein I, : 2 interactors Detail

Experiments performed 8 experiments Detail

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