BMRBj - BMREntryMaster

Found 1 Document (2.516 seconds)

BMRB: 5579

1MX7

Two Homologous Rat Cellular Retinol-binding Proteins Differ in Local Conformational Flexibility

Authors

Lu, J., Cistola, D.P., Li, E.

Assembly

rat cellular retinol-binding protein I, ligand free

Entity

1. rat cellular retinol-binding protein I, ligand free (polymer, Thiol state: all free), 134 monomers, 15702.69 Da Detail

PVDFNGYWKM LSNENFEEYL RALDVNVALR KIANLLKPDK EIVQDGDHMI IRTLSTFRNY IMDFQVGKEF EEDLTGIDDR KCMTTVSWDG DKLQCVQKGE KEGRGWTQWI EGDELHLEMR AEGVTCKQVF KKVH

Formula weight

15702.69 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.6 %, Completeness (bb): 98.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.6 % (1500 of 1620)	94.8 % (804 of 848)	88.6 % (553 of 624)	96.6 % (143 of 148)
Backbone	98.6 % (789 of 800)	98.6 % (272 of 276)	99.2 % (389 of 392)	97.0 % (128 of 132)
Sidechain	88.3 % (834 of 944)	93.0 % (532 of 572)	80.6 % (287 of 356)	93.8 % (15 of 16)
Aromatic	38.2 % (55 of 144)	65.3 % (47 of 72)	7.4 % (5 of 68)	75.0 % (3 of 4)
Methyl	99.3 % (133 of 134)	98.5 % (66 of 67)	100.0 % (67 of 67)

1. rat cellular retinol-binding protein I

PVDFNGYWKM LSNENFEEYL RALDVNVALR KIANLLKPDK EIVQDGDHMI IRTLSTFRNY IMDFQVGKEF EEDLTGIDDR KCMTTVSWDG DKLQCVQKGE KEGRGWTQWI EGDELHLEMR AEGVTCKQVF KKVH

Show sample condition

Sample

Temperature 298 (±1) K, pH 7.4 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹³C	DSS	methyl protons	external	indirect	0.25145
¹⁵N	ammonium chloride	ammonium nitrogen	external	direct	0.101329

Referencing offset: -0.18 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹³C	DSS	methyl protons	external	indirect	0.25145
¹⁵N	ammonium chloride	ammonium nitrogen	external	direct	0.101329

Referencing offset: -0.18 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹³C	DSS	methyl protons	external	indirect	0.25145
¹⁵N	ammonium chloride	ammonium nitrogen	external	direct	0.101329

Referencing offset: -0.1 ppm, Outliers: 6 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹³C	DSS	methyl protons	external	indirect	0.25145
¹⁵N	ammonium chloride	ammonium nitrogen	external	direct	0.101329

Referencing offset: 0.48 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 22 models in PDB: 1MX7, Strand ID: A Detail

Release date

2003-07-24

Citation

Two homologous rat cellular retinol-binding proteins differ in local conformational flexibility
Lu, J., Cistola, D.P., Li, E.
J. Mol. Biol. (2003), 330, 799-812, PubMed 12850148 , Abstract

Related entities 1. rat cellular retinol-binding protein I, ligand free, : 1 : 6 : 24 : 213 entities Detail

Interaction partners 1. rat cellular retinol-binding protein I, ligand free, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 8 experiments Detail

1 HNCO

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian UNITYPLUS (500 MHz)

#4 Model Varian UNITY (600 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	rat cellular retinol-binding protein I	[U-80% 2H; U-99% 15N]		1.0 mM

Sample #3

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
3	rat cellular retinol-binding protein I	[U-99% 15N]		1.0 mM

2 HNCACB

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian UNITYPLUS (500 MHz)

#4 Model Varian UNITY (600 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	rat cellular retinol-binding protein I	[U-80% 2H; U-99% 15N]		1.0 mM

Sample #3

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
3	rat cellular retinol-binding protein I	[U-99% 15N]		1.0 mM

3 CBCACONNH

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian UNITYPLUS (500 MHz)

#4 Model Varian UNITY (600 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	rat cellular retinol-binding protein I	[U-80% 2H; U-99% 15N]		1.0 mM

Sample #3

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
3	rat cellular retinol-binding protein I	[U-99% 15N]		1.0 mM

4 CBCACOCAHA

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian UNITYPLUS (500 MHz)

#4 Model Varian UNITY (600 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	rat cellular retinol-binding protein I	[U-80% 2H; U-99% 15N]		1.0 mM

Sample #3

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
3	rat cellular retinol-binding protein I	[U-99% 15N]		1.0 mM

5 1H-15N NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian UNITYPLUS (500 MHz)

#4 Model Varian UNITY (600 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	rat cellular retinol-binding protein I	[U-80% 2H; U-99% 15N]		1.0 mM

Sample #3

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
3	rat cellular retinol-binding protein I	[U-99% 15N]		1.0 mM

6 1H-15N TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian UNITYPLUS (500 MHz)

#4 Model Varian UNITY (600 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	rat cellular retinol-binding protein I	[U-80% 2H; U-99% 15N]		1.0 mM

Sample #3

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
3	rat cellular retinol-binding protein I	[U-99% 15N]		1.0 mM

7 HCCH-TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian UNITYPLUS (500 MHz)

#4 Model Varian UNITY (600 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	rat cellular retinol-binding protein I	[U-80% 2H; U-99% 15N]		1.0 mM

Sample #3

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
3	rat cellular retinol-binding protein I	[U-99% 15N]		1.0 mM

8 1H-13C NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian UNITYPLUS (500 MHz)

#4 Model Varian UNITY (600 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	rat cellular retinol-binding protein I	[U-99% 15N; U-13C]		1.0 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	rat cellular retinol-binding protein I	[U-80% 2H; U-99% 15N]		1.0 mM

Sample #3

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
3	rat cellular retinol-binding protein I	[U-99% 15N]		1.0 mM

nullKeywords cellular retinol-binding protein, vitamin A, NMR, structure, dynamics

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Found 1 Document (2.516 seconds)

BMRB: 5579

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. rat cellular retinol-binding protein I, ligand free, : 1 : 6 : 24 : 213 entities Detail

Interaction partners 1. rat cellular retinol-binding protein I, ligand free, : 2 interactors Detail

Experiments performed 8 experiments Detail

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