BMRB: 5585

Isolation, structure and activity of GID, a novel alpha4/7-conotoxin with an extended N-terminal sequence

Authors

Nicke, A.C., Loughnan, M.L., Millard, E.L., Alewood, P.F., Adams, D.J., Daly, N.L., Craik, D.J., Lewis, R.J.

Assembly

alpha-conotoxin GID

Entity

1. alpha-conotoxin GID (polymer, Thiol state: all disulfide bound), 19 monomers, 2190.420 Da Detail

IRDXCCSNPA CRVNNXHVC

Formula weight

2190.42 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	1:CYS11:SG
2	disulfide	sing	1:CYS6:SG	1:CYS19:SG

Source organism

Conus geographus

Exptl. method

NMR

Refine. method

Initial structures were calculated using torsion angle dynamics and structures were refined in a water shell using cartesian dynamics.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.5 %, Completeness: 84.9 %, Completeness (bb): 93.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	84.9 % (79 of 93)	84.9 % (79 of 93)
Backbone	93.9 % (31 of 33)	93.9 % (31 of 33)
Sidechain	80.0 % (48 of 60)	80.0 % (48 of 60)
Aromatic	0.0 % (0 of 2)	0.0 % (0 of 2)
Methyl	85.7 % (6 of 7)	85.7 % (6 of 7)

1. alpha-conotoxin GID

IRDXCCSNPA CRVNNXHVC

Show sample condition

Sample

Pressure 1 atm, Temperature 287 (±1) K, pH 2.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	alpha-conotoxin GID			2.5 mM
2	H2O			90 %
3	D2O			10 %

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Calculated from 20 models in PDB: 1MTQ, Strand ID: A Detail

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Release date

2002-11-12

Citation

Isolation, structure, and activity of GID, a novel alpha 4/7-conotoxin with an extended N-terminal sequence
Nicke, A.C., Loughnan, M.L., Millard, E.L., Alewood, P.F., Adams, D.J., Daly, N.L., Craik, D.J., Lewis, R.J.
J. Biol. Chem. (2003), 278, 3137-3144, PubMed 12419800 , DOI 10.1074/jbc.M210280200 , Abstract

Related entities 1. alpha-conotoxin GID, : 1 : 4 : 12 entities Detail

Experiments performed 4 experiments Detail