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BMRB: 5589

1NXI

Backbone and side chain 1H, 13C, and 15N chemical shift assignments for V. cholerae VC0424

Authors

Ramelot, T.A., Cort, J.R., Ni, S., Kennedy, M.A.

Assembly

VC0424

Entity

1. VC0424 (polymer, Thiol state: all free), 132 monomers, 15323.63 Da Detail

MSHQDDYLSV EELIEIQKEE TRDIIQALLE DGSDPDALYE IEHHLFAEDF DKLEKAAVEA FKMGFEVLEA EETEDEDGNK LLCFDATMQS ALDAKLIDEQ VEKLVNLAEK FDIIYDGWGT YYEGLEHHHH HH

Formula weight

15323.63 Da

Source organism

Vibrio cholerae

Exptl. method

NMR

Refine. method

distance geometry and simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 91.7 %, Completeness (bb): 93.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.7 % (1410 of 1538)	91.4 % (724 of 792)	91.8 % (557 of 607)	92.8 % (129 of 139)
Backbone	93.8 % (741 of 790)	93.7 % (252 of 269)	93.6 % (365 of 390)	94.7 % (124 of 131)
Sidechain	90.2 % (788 of 874)	90.2 % (472 of 523)	90.7 % (311 of 343)	62.5 % (5 of 8)
Aromatic	73.0 % (108 of 148)	73.0 % (54 of 74)	72.6 % (53 of 73)	100.0 % (1 of 1)
Methyl	100.0 % (142 of 142)	100.0 % (71 of 71)	100.0 % (71 of 71)

1. VC0424

MSHQDDYLSV EELIEIQKEE TRDIIQALLE DGSDPDALYE IEHHLFAEDF DKLEKAAVEA FKMGFEVLEA EETEDEDGNK LLCFDATMQS ALDAKLIDEQ VEKLVNLAEK FDIIYDGWGT YYEGLEHHHH HH

Show sample condition

Sample

Temperature 298 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	VC0424	[U-15N; U-13C]	1.0 ~ 2.0 mM
2	Tris buffer		20 mM
3	NaCl		500 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.46 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.46 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.05 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1NXI, Strand ID: A Detail

Release date

2003-06-22

Citation

Solution structure of Vibrio cholorae protein VC0424: A variation of the Ferredoxin-like fold
Ramelot, T.A., Ni, S., Goldsmith-Fischman, S., Cort, J.R., Honig, B., Kennedy, M.A.
Protein Sci. (2003), 12, 1562-1566, PubMed , DOI:

Related entities 1. VC0424, : 1 : 21 entities Detail

Experiments performed 20 experiments Detail

1 1H-15N HSQC