BMRB: 5604

Solution structure of the N-terminal domain of ZntA in the apo- and Zn(II) forms

Authors

Banci, L., Bertini, I., Ciofi-Baffoni, S., Finney, L.A., Outten, C.E., O'Halloran, T.V.

Assembly

ZNTA (E.C.3.6.3.3/E.C.3.6.3.5)

Entity

1. ZNTA (E.C.3.6.3.3/E.C.3.6.3.5) (polymer, Thiol state: all free), 73 monomers, 7930.789 Da Detail

SGTRYSWKVS GMDCAACARK VENAVRQLAG VNQVQVLFAT EKLVVDADND IRAQVESALQ KAGYSLRDEQ AAE

Formula weight

7930.789 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 81.9 %, Completeness (bb): 97.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	81.9 % (407 of 497)	80.7 % (334 of 414)	88.0 % (73 of 83)
Backbone	97.3 % (217 of 223)	96.7 % (145 of 150)	98.6 % (72 of 73)
Sidechain	69.3 % (190 of 274)	71.6 % (189 of 264)	10.0 % (1 of 10)
Aromatic	70.0 % (14 of 20)	68.4 % (13 of 19)	100.0 % (1 of 1)
Methyl	88.6 % (39 of 44)	88.6 % (39 of 44)

1. p-type ATPase

SGTRYSWKVS GMDCAACARK VENAVRQLAG VNQVQVLFAT EKLVVDADND IRAQVESALQ KAGYSLRDEQ AAE

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	p-type ATPase	[U-15N]		1.5 mM
2	phosphate buffer			100 mM
3	H2O			90 %
4	D2O			10 %

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Protein Blocks Logo

Calculated from 30 models in PDB: 1MWY, Strand ID: A Detail

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Release date

2003-03-23

Citation

A new zinc-protein coordination site in intracellular metal trafficking: solution structure of the Apo and Zn(II) forms of ZntA(46-118)
Banci, L., Bertini, I., Ciofi-Baffoni, S., Finney, L.A., Outten, C.E., O'Halloran, T.V.
J. Mol. Biol. (2002), 323, 883-897, PubMed 12417201 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. ZNTA (E.C.3.6.3.3/E.C.3.6.3.5), : 1 : 3 : 2 : 125 entities Detail

Interaction partners 1. ZNTA (E.C.3.6.3.3/E.C.3.6.3.5), : 3 interactors Detail

Experiments performed 4 experiments Detail