BMRB: 5607

NMR Structure of the Extended Second Transmembrane Domain of the Human Neuronal Glycine Receptor alpha1 Subunit in SDS Micelles

Authors

Yushmanov, V.E., Mandal, P.K., Liu, Z., Tang, P., Xu, Y.

Assembly

Human Neuronal Glycine Receptor alpha-1 Chain TM2

Entity

1. Human Neuronal Glycine Receptor alpha-1 Chain TM2 (polymer, Thiol state: not present), 28 monomers, 2801.223 × 4 Da Detail

APARVGLGIT TVLTMTTQSS GSRASLPK

Total weight

11204.892 Da

Max. entity weight

2801.223 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 82.4 %, Completeness (bb): 96.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	82.4 % (145 of 176)	80.5 % (120 of 149)	92.6 % (25 of 27)
Backbone	96.4 % (80 of 83)	96.5 % (55 of 57)	96.2 % (25 of 26)
Sidechain	69.9 % (65 of 93)	70.7 % (65 of 92)	0.0 % (0 of 1)
Methyl	85.0 % (17 of 20)	85.0 % (17 of 20)

1. Human Neuronal Glycine Receptor alpha-1 Chain TM2

APARVGLGIT TVLTMTTQSS GSRASLPK

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Sample

Pressure 1 atm, Temperature 303 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human Neuronal Glycine Receptor alpha-1 Chain TM2	[U-15N]		2 mM
2	sodium dodecyl sulfate			300 mM
3	H2O			90 %
4	D2O			10 %

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Calculated from 20 models in PDB: 1MOT, Strand ID: A Detail

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Coupling constant

19 J values in 1 lists
Pressure 1 atm, Temperature 303 (±1) K, pH 5.0 (±0.2) Detail

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Pressure 1 atm, Temperature 303 (±1) K, pH 5.0 (±0.2)

List #1 coupling_constants_set_1

Release date

2003-05-13

Citation

NMR structure and backbone dynamics of the extended second transmembrane domain of the human neuronal glycine receptor alpha1 subunit
Yushmanov, V.E., Mandal, P.K., Liu, Z., Tang, P., Xu, Y.
Biochemistry (2003), 42, 3989-3995, PubMed 12667090 , DOI 10.1021/bi026767g , Abstract

Related entities 1. Human Neuronal Glycine Receptor alpha-1 Chain TM2, : 1 : 20 : 2 : 19 : 152 entities Detail

Interaction partners 1. Human Neuronal Glycine Receptor alpha-1 Chain TM2, : 2 interactors Detail

Experiments performed 7 experiments Detail