BMRB: 5612

Structure of the Plant Defensin PhD1 from Petunia Hybrida

Authors

Janssen, B.J.C., Schirra, H.J., Lay, F.T., Anderson, M.A., Craik, D.J.

Assembly

floral defensin-like protein 1

Entity

1. floral defensin-like protein 1 (polymer, Thiol state: all disulfide bound), 47 monomers, 5211.245 Da Detail

ATCKAECPTW DSVCINKKPC VACCKKAKFS DGHCSKILRR CLCTKEC

Formula weight

5211.245 Da

Entity Connection

disulfide 5 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS3:SG	1:CYS47:SG
2	disulfide	sing	1:CYS7:SG	1:CYS23:SG
3	disulfide	sing	1:CYS14:SG	1:CYS34:SG
4	disulfide	sing	1:CYS20:SG	1:CYS41:SG
5	disulfide	sing	1:CYS24:SG	1:CYS43:SG

Source organism

Petunia x hybrida

Exptl. method

NMR

Refine. method

Torsion angle simulated annealing protocol, incorporated in CNS.

Data set

coupling_constants

Protein Blocks Logo

Calculated from 20 models in PDB: 1N4N, Strand ID: A Detail

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Coupling constant

13 J values in 1 lists
Field strength (¹H) 750 MHz, Pressure 1 atm, Temperature 310 K, pH 3.1 Detail

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Pressure 1 atm, Temperature 310 K, pH 3.1

Experiment name 2D NOESY, E-COSY, DQF-COSY

List #1 coupling_constants_1, Field strength (¹H) 750 MHz

Release date

2002-12-01

Citation

Structure of Petunia hybrida defensin 1, a novel plant defensin with five disulfide bonds
Janssen, B.J.C., Schirra, H.J., Lay, F.T., Anderson, M.A., Craik, D.J.
Biochemistry (2003), 42, 8214-8222, PubMed 12846570 , DOI 10.1021/bi034379o , Abstract

Related entities 1. floral defensin-like protein 1, : 1 : 1 : 100 entities Detail

Experiments performed 3 experiments Detail