BMRB: 5614

Sheared A(anti)-A(anti) Pairs in a Destabilizing 2x2 Internal Loop: The NMR Structure of 5'(rGGCAAGCCU)2

Authors

Znosko, B.M., Burkard, M.E., Schroeder, S.J., Krugh, T.R., Turner, D.H.

Assembly

5'-R(*GP*GP*CP*AP*AP*GP*CP*CP*U)-3'

Entity

1. 5'-R(*GP*GP*CP*AP*AP*GP*CP*CP*U)-3' (polymer, Thiol state: not present), 9 monomers, 2933.755 × 2 Da Detail

GGCAAGCCU

Total weight

5867.51 Da

Max. entity weight

2933.755 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.9 %, Completeness: 88.9 %, Completeness (bb): 88.9 % Detail

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Polymer type: polyribonucleotide

	Total	31P
All	88.9 % (8 of 9)	88.9 % (8 of 9)
Suger, PO4	88.9 % (8 of 9)	88.9 % (8 of 9)

1. dlRNA

GGCAAGCCU

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Sample

Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	dlRNA			1 mM
2	NaCl			80 mM
3	phosphate buffer			10 mM
4	EDTA			0.5 mM
5	H2O			90 %
6	D2O			10 %

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Release date

2002-12-26

Citation

Sheared Aanti.Aanti base pairs in a destabilizing 2 x 2 internal loop: the NMR structure of 5'(rGGCAAGCCU)2
Znosko, B.M., Burkard, M.E., Schroeder, S.J., Krugh, T.R., Turner, D.H.
Biochemistry (2002), 41, 14969-14977, PubMed 12475246 , DOI: , Abstract

Experiments performed 1 experiments Detail