BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	76.9 % (821 of 1068)	80.6 % (452 of 561)	69.1 % (288 of 417)	90.0 % (81 of 90)
Backbone	76.4 % (388 of 508)	90.1 % (155 of 172)	63.0 % (160 of 254)	89.0 % (73 of 82)
Sidechain	79.0 % (507 of 642)	76.3 % (297 of 389)	82.4 % (202 of 245)	100.0 % (8 of 8)
Aromatic	63.1 % (82 of 130)	66.2 % (43 of 65)	57.4 % (35 of 61)	100.0 % (4 of 4)
Methyl	95.6 % (65 of 68)	97.1 % (33 of 34)	94.1 % (32 of 34)

1. WW domain pair of pre-mRNA processing protein 40

MKHHHHHHPM GMSIWKEAKD ASGRIYYYNT LTKKSTWEKP KELISQEELL LRENGWKAAK TADGKVYYYN PTTRETSWTI PAFEKK

Show sample condition

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	WW domain pair of pre-mRNA processing protein 40	[U-100% 15N]		1.5 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	WW domain pair of pre-mRNA processing protein 40	[U-100% 13C; U-100% 15N]		1.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.81 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 2.33 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.81 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 2.33 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.81 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 7 models in PDB: 1O6W, Strand ID: A Detail

Release date

2002-12-26

Citation

Solution structure and ligand recognition of the WW domain pair of the yeast splicing factor Prp40
Wiesner, S., Stier, G., Sattler, M., Macias, M.J.
J. Mol. Biol. (2002), 324, 807-822, PubMed 12460579 , DOI: , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P33203 released on 1994-02-01
Title PRP40_YEAST Entity Pre-mRNA-processing protein PRP40

Related entities 1. WW domain pair of S.cerevisiae pre-mRNA processing protein 40, : 1 : 4 : 116 entities Detail

Interaction partners 1. WW domain pair of S.cerevisiae pre-mRNA processing protein 40, : 142 interactors Detail

More details for 142 interactors identified by 9 citations

Experiments performed 11 experiments Detail

1 HN-HSQC

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	WW domain pair of pre-mRNA processing protein 40	[U-100% 15N]		1.5 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	WW domain pair of pre-mRNA processing protein 40	[U-100% 13C; U-100% 15N]		1.5 mM

2 HNCA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	WW domain pair of pre-mRNA processing protein 40	[U-100% 15N]		1.5 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	WW domain pair of pre-mRNA processing protein 40	[U-100% 13C; U-100% 15N]		1.5 mM

3 CBCANH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	WW domain pair of pre-mRNA processing protein 40	[U-100% 15N]		1.5 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	WW domain pair of pre-mRNA processing protein 40	[U-100% 13C; U-100% 15N]		1.5 mM

4 CBCA(CO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	WW domain pair of pre-mRNA processing protein 40	[U-100% 15N]		1.5 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	WW domain pair of pre-mRNA processing protein 40	[U-100% 13C; U-100% 15N]		1.5 mM

5 HNHA-J

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	WW domain pair of pre-mRNA processing protein 40	[U-100% 15N]		1.5 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	WW domain pair of pre-mRNA processing protein 40	[U-100% 13C; U-100% 15N]		1.5 mM

6 HC-HSQC

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	WW domain pair of pre-mRNA processing protein 40	[U-100% 15N]		1.5 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	WW domain pair of pre-mRNA processing protein 40	[U-100% 13C; U-100% 15N]		1.5 mM

7 HC(CO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	WW domain pair of pre-mRNA processing protein 40	[U-100% 15N]		1.5 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	WW domain pair of pre-mRNA processing protein 40	[U-100% 13C; U-100% 15N]		1.5 mM

8 C(CO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	WW domain pair of pre-mRNA processing protein 40	[U-100% 15N]		1.5 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	WW domain pair of pre-mRNA processing protein 40	[U-100% 13C; U-100% 15N]		1.5 mM

9 HC(C)H-TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	WW domain pair of pre-mRNA processing protein 40	[U-100% 15N]		1.5 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	WW domain pair of pre-mRNA processing protein 40	[U-100% 13C; U-100% 15N]		1.5 mM

10 13C-NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	WW domain pair of pre-mRNA processing protein 40	[U-100% 15N]		1.5 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	WW domain pair of pre-mRNA processing protein 40	[U-100% 13C; U-100% 15N]		1.5 mM

11 15N-NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	WW domain pair of pre-mRNA processing protein 40	[U-100% 15N]		1.5 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	WW domain pair of pre-mRNA processing protein 40	[U-100% 13C; U-100% 15N]		1.5 mM

nullKeywords CTD repeat, NMR structure, proline-rich, Prp40, WW domain

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BMRB: 5616

Sample #1

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. WW domain pair of S.cerevisiae pre-mRNA processing protein 40, : 1 : 4 : 116 entities Detail

Interaction partners 1. WW domain pair of S.cerevisiae pre-mRNA processing protein 40, : 142 interactors Detail

Experiments performed 11 experiments Detail

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