BMRB: 5617

1H chemical shift assignments for the aticarcinogenic Bowman Birk inhibitor isolated from snail medic seeds (Medicago Scutellata)

Authors

Catalano, M., Ragona, L., Molinari, H., Tava, A., Zetta, L.

Assembly

Bowman-Birk inhibitor from snail medic seeds (Medicago Scutellata) (MSTI)

Entity

1. Bowman-Birk inhibitor from snail medic seeds (Medicago Scutellata) (MSTI) (polymer, Thiol state: all disulfide bound), 62 monomers, 6931.951 Da Detail

TKSTTTACCD FCPCTRSIPP QCQCTDVREK CHSACKSCLC TRSFPPQCRC YDITDFCYPS CS

Formula weight

6931.951 Da

Entity Connection

disulfide 7 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS8:SG	1:CYS61:SG
2	disulfide	sing	1:CYS9:SG	1:CYS24:SG
3	disulfide	sing	1:CYS12:SG	1:CYS57:SG
4	disulfide	sing	1:CYS14:SG	1:CYS22:SG
5	disulfide	sing	1:CYS31:SG	1:CYS38:SG
6	disulfide	sing	1:CYS35:SG	1:CYS50:SG
7	disulfide	sing	1:CYS40:SG	1:CYS48:SG

Source organism

Medicago scutellata

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 95.2 %, Completeness (bb): 97.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	95.2 % (334 of 351)	95.2 % (334 of 351)
Backbone	97.5 % (115 of 118)	97.5 % (115 of 118)
Sidechain	94.0 % (219 of 233)	94.0 % (219 of 233)
Aromatic	92.0 % (23 of 25)	92.0 % (23 of 25)
Methyl	100.0 % (18 of 18)	100.0 % (18 of 18)

1. Bowman Birk inhibitor

TKSTTTACCD FCPCTRSIPP QCQCTDVREK CHSACKSCLC TRSFPPQCRC YDITDFCYPS CS

Show sample condition

Sample

Pressure 1 atm, Temperature 300 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Bowman Birk inhibitor			1 mM
2	phosphate buffer			20 mM

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Protein Blocks Logo

Calculated from 15 models in PDB: 1MVZ, Strand ID: A Detail

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Release date

2002-12-08

Citation

Anticarcinogenic Bowman Birk inhibitor isolated from snail medic seeds (Medicago scutellata): solution structure and analysis of self-association behavior
Catalano, M., Ragona, L., Molinari, H., Tava, A., Zetta, L.
Biochemistry (2003), 42, 2836-2846, PubMed 12627949 , DOI 10.1021/bi020576w , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Bowman-Birk inhibitor from snail medic seeds (Medicago Scutellata) (MSTI), : 1 : 2 : 88 entities Detail

Experiments performed 3 experiments Detail