BMRB: 5621

Assignment for yoag_ecoli

Authors

Wu, B., Pineda-Lucena, A., Yee, A., John, C., Kennedy, M., Arrowsmith, C.

Assembly

yoag ecoli

Entity

1. yoag ecoli (polymer, Thiol state: all other bound), 60 monomers, 6608.218 × 2 Da Detail

MGKATYTVTV TNNSNGVSVD YETETPMTLL VPEVAAEVIK DLVNTVRSYD TENEHDVCGW

Total weight

13216.436 Da

Max. entity weight

6608.218 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 86.3 %, Completeness (bb): 83.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.3 % (561 of 650)	96.3 % (315 of 327)	71.0 % (184 of 259)	96.9 % (62 of 64)
Backbone	83.4 % (297 of 356)	98.3 % (119 of 121)	68.4 % (121 of 177)	98.3 % (57 of 58)
Sidechain	91.2 % (320 of 351)	95.1 % (196 of 206)	85.6 % (119 of 139)	83.3 % (5 of 6)
Aromatic	37.5 % (15 of 40)	70.0 % (14 of 20)	0.0 % (0 of 19)	100.0 % (1 of 1)
Methyl	100.0 % (80 of 80)	100.0 % (40 of 40)	100.0 % (40 of 40)

1. yoag ecoli

MGKATYTVTV TNNSNGVSVD YETETPMTLL VPEVAAEVIK DLVNTVRSYD TENEHDVCGW

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	yoag_ecoli	[U-13C; U-15N]		1.0 mM
2	NaCl			450 mM
3	Na2PO4			25 mM

Hide sample codition

LACS Plot; CA

Referencing offset: -0.11 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.11 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.12 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 19 models in PDB: 1NEI, Strand ID: A, B Detail

---

Release date

2002-12-26

Citation

Solution structure of hypothetical protein dimer encoded by the Yoag gene from Escherichia coli"
Wu, B., Pineda-Lucena, A., Yee, A., John, C., Kennedy, M., Arrowsmith, C.

Related entities 1. yoag ecoli, : 1 : 2 entities Detail

Experiments performed 10 experiments Detail