BMRB: 5622

Structural and Functional Characterization of a Thioredoxin-like Protein from methanobacterium thermoautotrophicum (Delta H)

Authors

Amegbey, G.YAO., Monzavi, H., Habibi-Nazad, B., Bhattacharyya, S., Wishart, D.S.

Assembly

Thioredoxin-like Protein from Methanobacterium Thermoautotrophicum

Entity

1. Thioredoxin-like Protein from Methanobacterium Thermoautotrophicum (polymer, Thiol state: all free), 85 monomers, 9480.875 Da Detail

MVVNIEVFTS PTCPYCPMAI EVVDEAKKEF GDKIDVEKID IMVDREKAIE YGLMAVPAIA INGVVRFVGA PSREELFEAI NDEME

Formula weight

9480.875 Da

Source organism

Methanothermobacter thermautotrophicus

Exptl. method

NMR

Refine. method

distance geometry and simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 81.1 %, Completeness (bb): 97.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	81.1 % (792 of 977)	91.9 % (464 of 505)	63.8 % (248 of 389)	96.4 % (80 of 83)
Backbone	97.4 % (487 of 500)	98.2 % (166 of 169)	96.0 % (241 of 251)	100.0 % (80 of 80)
Sidechain	68.6 % (383 of 558)	88.7 % (298 of 336)	38.8 % (85 of 219)	0.0 % (0 of 3)
Aromatic	46.4 % (26 of 56)	92.9 % (26 of 28)	0.0 % (0 of 28)
Methyl	55.6 % (60 of 108)	87.0 % (47 of 54)	24.1 % (13 of 54)

1. Thioredoxin from M. thermoautotrophicum

MVVNIEVFTS PTCPYCPMAI EVVDEAKKEF GDKIDVEKID IMVDREKAIE YGLMAVPAIA INGVVRFVGA PSREELFEAI NDEME

Show sample condition

Sample

Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Thioredoxin from M. thermoautotrophicum	[U-13C; U-15N]		1 mM
2	NaH2PO4			50 mM
3	NaCl			100 mM

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LACS Plot; CA

Referencing offset: -0.53 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: -0.53 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CO

Referencing offset: -0.02 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 1NHO, Strand ID: A Detail

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Release date

2003-08-07

Citation

Structural and functional characterization of a thioredoxin-like protein (Mt0807) from Methanobacterium thermoautotrophicum
Amegbey, G.Y., Monzavi, H., Habibi-Nazhad, B., Bhattacharyya, S., Wishart, D.S.
Biochemistry (2003), 42, 8001-8010, PubMed 12834352 , DOI 10.1021/bi030021g , Abstract

Related entities 1. Thioredoxin-like Protein from Methanobacterium Thermoautotrophicum, : 1 : 1 : 167 entities Detail

Experiments performed 7 experiments Detail