BMRBj - BMREntryMaster

Found 1 Document (6.041 seconds)

BMRB: 5624

1ND9

A Conserved Structural Motif at the N-terminal of Bacterial Translation Initiation Factor IF2

Authors

Laursen, B.S., Mortensen, K.K., Sperling-Petersen, H.U., Hoffman, D.W.

Assembly

translation initiation factor IF2

Entity

1. translation initiation factor IF2 (polymer, Thiol state: not present), 156 monomers, 17523.14 Da Detail

TDVTIKTLAA ERQTSVERLV QQFADAGIRK SADDSVSAQE KQTLIDHLNQ KNSGPDKLTL QRKTRSTLNI PGTGGKSKSV QIEVRKKRTF VKRDPQEAER LAAEEQAQRE AEEQARREAE ESAKREAQQK AEREAAEQAK REAAEQAKRE AAEKDK

Formula weight

17523.14 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 78.8 %, Completeness: 67.6 %, Completeness (bb): 76.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	67.6 % (1213 of 1795)	70.5 % (675 of 958)	63.2 % (420 of 665)	68.6 % (118 of 172)
Backbone	76.7 % (713 of 930)	76.8 % (241 of 314)	77.3 % (358 of 463)	74.5 % (114 of 153)
Sidechain	60.8 % (618 of 1016)	67.4 % (434 of 644)	51.0 % (180 of 353)	21.1 % (4 of 19)
Aromatic	50.0 % (12 of 24)	100.0 % (12 of 12)	0.0 % (0 of 12)
Methyl	79.1 % (117 of 148)	89.2 % (66 of 74)	68.9 % (51 of 74)

1. Translation factor IF2

TDVTIKTLAA ERQTSVERLV QQFADAGIRK SADDSVSAQE KQTLIDHLNQ KNSGPDKLTL QRKTRSTLNI PGTGGKSKSV QIEVRKKRTF VKRDPQEAER LAAEEQAQRE AEEQARREAE ESAKREAQQK AEREAAEQAK REAAEQAKRE AAEKDK

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Translation factor IF2			1.5 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Translation factor IF2	[U-15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Translation factor IF2	[U-15N; U-13C]		1.5 mM

Sample #4

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Translation factor IF2	[U-15N]-Lys		1.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.15 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.15 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.62 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1ND9, Strand ID: A Detail

Release date

2003-02-27

Citation

A conserved structural motif at the N terminus of bacterial translation initiation factor IF2
Laursen, B.S., Mortensen, K.K., Sperling-Petersen, H.U., Hoffman, D.W.
J. Biol. Chem. (2003), 278, 16320-16328, PubMed 12600987 , DOI 10.1074/jbc.M212960200 , Abstract

Related entities 1. translation initiation factor IF2, : 7 : 1 : 1 : 52 entities Detail

Interaction partners 1. translation initiation factor IF2, : 38 interactors Detail

More details for 38 interactors identified by 4 citations

Experiments performed 9 experiments Detail

1 HNCA

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Translation factor IF2			1.5 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Translation factor IF2	[U-15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Translation factor IF2	[U-15N; U-13C]		1.5 mM

Sample #4

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Translation factor IF2	[U-15N]-Lys		1.5 mM

2 HNCO

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Translation factor IF2			1.5 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Translation factor IF2	[U-15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Translation factor IF2	[U-15N; U-13C]		1.5 mM

Sample #4

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Translation factor IF2	[U-15N]-Lys		1.5 mM

3 HNCACB

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Translation factor IF2			1.5 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Translation factor IF2	[U-15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Translation factor IF2	[U-15N; U-13C]		1.5 mM

Sample #4

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Translation factor IF2	[U-15N]-Lys		1.5 mM

4 HN(CO)CACB

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Translation factor IF2			1.5 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Translation factor IF2	[U-15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Translation factor IF2	[U-15N; U-13C]		1.5 mM

Sample #4

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Translation factor IF2	[U-15N]-Lys		1.5 mM

5 HACACBCO

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Translation factor IF2			1.5 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Translation factor IF2	[U-15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Translation factor IF2	[U-15N; U-13C]		1.5 mM

Sample #4

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Translation factor IF2	[U-15N]-Lys		1.5 mM

6 15N-edited HSQC TOCSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Translation factor IF2			1.5 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Translation factor IF2	[U-15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Translation factor IF2	[U-15N; U-13C]		1.5 mM

Sample #4

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Translation factor IF2	[U-15N]-Lys		1.5 mM

7 13C-edited HCCH-TOCSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Translation factor IF2			1.5 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Translation factor IF2	[U-15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Translation factor IF2	[U-15N; U-13C]		1.5 mM

Sample #4

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Translation factor IF2	[U-15N]-Lys		1.5 mM

8 2QF-COSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Translation factor IF2			1.5 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Translation factor IF2	[U-15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Translation factor IF2	[U-15N; U-13C]		1.5 mM

Sample #4

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Translation factor IF2	[U-15N]-Lys		1.5 mM

9 TOCSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Translation factor IF2			1.5 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Translation factor IF2	[U-15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Translation factor IF2	[U-15N; U-13C]		1.5 mM

Sample #4

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Translation factor IF2	[U-15N]-Lys		1.5 mM

nullKeywords IF2, translation factor, initiation, infb

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Found 1 Document (6.041 seconds)

BMRB: 5624

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Chemical shift reference

Related entities 1. translation initiation factor IF2, : 7 : 1 : 1 : 52 entities Detail

Interaction partners 1. translation initiation factor IF2, : 38 interactors Detail

Experiments performed 9 experiments Detail

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