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BMRB: 5632

1NA2

Solution structure of the p2b hairpin from human telomerase RNA

Authors

Theimer, C.A., Finger, L.D., Trantirek, L., Feigon, J.

Assembly

telomerase RNA p2b hairpin

Entity

1. telomerase RNA p2b hairpin (polymer, Thiol state: not present), 30 monomers, 9512.507 Da Detail

GGGCUGUUUU UCUCGCUGAC UUUCAGCCCC

Formula weight

9512.507 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.5 %, Completeness (bb): 96.7 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	92.5 % (455 of 492)	93.7 % (254 of 271)	92.1 % (187 of 203)	77.8 % (14 of 18)
Suger, PO4	96.7 % (319 of 330)	96.1 % (173 of 180)	97.3 % (146 of 150)
Nucleobase	84.0 % (136 of 162)	89.0 % (81 of 91)	77.4 % (41 of 53)	77.8 % (14 of 18)
Aromatic	85.9 % (122 of 142)	94.4 % (67 of 71)	77.4 % (41 of 53)	77.8 % (14 of 18)

1. human telomerase RNA

GGGCUGUUUU UCUCGCUGAC UUUCAGCCCC

Show sample condition

Sample #1

Pressure 1 atm, Temperature 278 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human telomerase RNA			1 mM
2	Na phosphate			10 mM
3	KCl			200 mM
4	NaN3			0.2 %
5	EDTA			50 uM
6	H2O			95 %
7	D2O			5 %

Sample #2

Pressure 1 atm, Temperature 278 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
8	human telomerase RNA	[U-100% 13C; U-100% 15N]		1 mM
9	Na phosphate			10 mM
10	KCl			200 mM
11	NaN3			0.2 %
12	EDTA			50 uM
13	H2O			95 %
14	D2O			100 %

Sample #3

Pressure 1 atm, Temperature 278 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
15	human telomerase RNA	[U-100% 13C; U-100% 15N]		1 mM

Release date

2004-03-06

Citation

Mutations linked to dyskeratosis congenita cause changes in the structural equilibrium in telomerase RNA
Theimer, C.A., Finger, L.D., Trantirek, L., Feigon, J.
Proc. Natl. Acad. Sci. U. S. A. (2003), 100, 449-454, PubMed 12525685 , DOI 10.1073/pnas.242720799 , Abstract

Related entities 1. telomerase RNA p2b hairpin, : 1 : 3 entities Detail

Experiments performed 4 experiments Detail

1 2D NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human telomerase RNA			1 mM
2	Na phosphate			10 mM
3	KCl			200 mM
4	NaN3			0.2 %
5	EDTA			50 uM
6	H2O			95 %
7	D2O			5 %

Sample #2

Pressure 1 atm, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
8	human telomerase RNA	[U-100% 13C; U-100% 15N]		1 mM
9	Na phosphate			10 mM
10	KCl			200 mM
11	NaN3			0.2 %
12	EDTA			50 uM
13	H2O			95 %
14	D2O			100 %

Sample #3

Pressure 1 atm, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
15	human telomerase RNA	[U-100% 13C; U-100% 15N]		1 mM

2 DQF-COSY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human telomerase RNA			1 mM
2	Na phosphate			10 mM
3	KCl			200 mM
4	NaN3			0.2 %
5	EDTA			50 uM
6	H2O			95 %
7	D2O			5 %

Sample #2

Pressure 1 atm, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
8	human telomerase RNA	[U-100% 13C; U-100% 15N]		1 mM
9	Na phosphate			10 mM
10	KCl			200 mM
11	NaN3			0.2 %
12	EDTA			50 uM
13	H2O			95 %
14	D2O			100 %

Sample #3

Pressure 1 atm, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
15	human telomerase RNA	[U-100% 13C; U-100% 15N]		1 mM

3 3D 13C-separated NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human telomerase RNA			1 mM
2	Na phosphate			10 mM
3	KCl			200 mM
4	NaN3			0.2 %
5	EDTA			50 uM
6	H2O			95 %
7	D2O			5 %

Sample #2

Pressure 1 atm, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
8	human telomerase RNA	[U-100% 13C; U-100% 15N]		1 mM
9	Na phosphate			10 mM
10	KCl			200 mM
11	NaN3			0.2 %
12	EDTA			50 uM
13	H2O			95 %
14	D2O			100 %

Sample #3

Pressure 1 atm, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
15	human telomerase RNA	[U-100% 13C; U-100% 15N]		1 mM

4 3D 13C NOESY-HMQC

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human telomerase RNA			1 mM
2	Na phosphate			10 mM
3	KCl			200 mM
4	NaN3			0.2 %
5	EDTA			50 uM
6	H2O			95 %
7	D2O			5 %

Sample #2

Pressure 1 atm, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
8	human telomerase RNA	[U-100% 13C; U-100% 15N]		1 mM
9	Na phosphate			10 mM
10	KCl			200 mM
11	NaN3			0.2 %
12	EDTA			50 uM
13	H2O			95 %
14	D2O			100 %

Sample #3

Pressure 1 atm, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
15	human telomerase RNA	[U-100% 13C; U-100% 15N]		1 mM

nullKeywords hairpin, narrow minor groove, NMR, pentaloop, RNA, telomerase, U-C base pair, U tracts, U-U base pair