BMRBj - BMREntryMaster

Found 1 Document (1.821 seconds)

BMRB: 5654

1MPE

A Protein Contorsionist-Core Mutations Switch Monomeric Protein GB1 into an Intertwined Tetramer

Authors

Frank, M.K., Dyda, F., Dobrodumov, A., Gronenborn, A.M.

Assembly

Immunoglobulin G binding protein G

Entity

1. Immunoglobulin G binding protein G (polymer, Thiol state: not present), 56 monomers, 6296.879 × 4 Da Detail

MQYKVILNGK TLKGETTTEA VDAATFEKVV KQFFNDNGVD GEWTYDDATK TFTVTE

Total weight

25187.516 Da

Max. entity weight

6296.879 Da

Source organism

Streptococcus sp.

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.4 %, Completeness (bb): 97.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.4 % (583 of 645)	87.2 % (286 of 328)	92.9 % (237 of 255)	96.8 % (60 of 62)
Backbone	97.3 % (327 of 336)	97.4 % (113 of 116)	97.6 % (160 of 164)	96.4 % (54 of 56)
Sidechain	85.0 % (307 of 361)	81.6 % (173 of 212)	89.5 % (128 of 143)	100.0 % (6 of 6)
Aromatic	61.8 % (42 of 68)	61.8 % (21 of 34)	60.6 % (20 of 33)	100.0 % (1 of 1)
Methyl	96.9 % (62 of 64)	96.9 % (31 of 32)	96.9 % (31 of 32)

1. mmunoglobulin G binding protein G

MQYKVILNGK TLKGETTTEA VDAATFEKVV KQFFNDNGVD GEWTYDDATK TFTVTE

Show sample condition

Sample

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
1	mmunoglobulin G binding protein G	[U-15N; U-13C]	3.5 mM
2	sodium phosphate		50 mM
3	sodium azide		0.02 %
4	H2O		90 %
5	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	TSP	methyl protons	null	indirect
¹H	na	methyl protons	null	null
¹⁵N	TSP	methyl protons	null	indirect

Referencing offset: -0.4 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	TSP	methyl protons	null	indirect
¹H	na	methyl protons	null	null
¹⁵N	TSP	methyl protons	null	indirect

Referencing offset: -0.4 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	TSP	methyl protons	null	indirect
¹H	na	methyl protons	null	null
¹⁵N	TSP	methyl protons	null	indirect

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	TSP	methyl protons	null	indirect
¹H	na	methyl protons	null	null
¹⁵N	TSP	methyl protons	null	indirect

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 21 models in PDB: 1MPE, Strand ID: A, B, C, D Detail

Coupling constant

44 J values in 1 lists
Pressure 1 atm, Temperature 313 K, pH 5.45 Detail

Release date

2003-08-07

Citation

Core mutations switch monomeric protein GB1 into an intertwined tetramer
Frank, M.K., Dyda, F., Dobrodumov, A., Gronenborn, A.M.
Nat. Struct. Biol. (2002), 9, 877-885, PubMed 12379842 , DOI 10.1038/nsb854 , Abstract

Related entities 1. Immunoglobulin G binding protein G, : 1 : 1 : 1 : 102 entities Detail

Interaction partners 1. Immunoglobulin G binding protein G, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 6 experiments Detail

1 3D 15N-separated NOESY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
1	mmunoglobulin G binding protein G	[U-15N; U-13C]	3.5 mM
2	sodium phosphate		50 mM
3	sodium azide		0.02 %
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
6	mmunoglobulin G binding protein G	[U-15N]	2.89 mM
7	sodium phosphate		50 mM
8	sodium azide		0.02 %
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
11	mmunoglobulin G binding protein G	[U-15N; U-13C]	3.5 mM
12	sodium phosphate		50 mM
13	sodium azide		0.02 %
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
15	mmunoglobulin G binding protein G		0.7 mM
16	mmunoglobulin G binding protein G	[U-15N; U-13C]	0.7 mM
17	sodium phosphate		50 mM
18	sodium azide		0.02 %
19	D2O		100 %

2 HNHA

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
1	mmunoglobulin G binding protein G	[U-15N; U-13C]	3.5 mM
2	sodium phosphate		50 mM
3	sodium azide		0.02 %
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
6	mmunoglobulin G binding protein G	[U-15N]	2.89 mM
7	sodium phosphate		50 mM
8	sodium azide		0.02 %
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
11	mmunoglobulin G binding protein G	[U-15N; U-13C]	3.5 mM
12	sodium phosphate		50 mM
13	sodium azide		0.02 %
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
15	mmunoglobulin G binding protein G		0.7 mM
16	mmunoglobulin G binding protein G	[U-15N; U-13C]	0.7 mM
17	sodium phosphate		50 mM
18	sodium azide		0.02 %
19	D2O		100 %

3 3D 13C-separated NOESY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
1	mmunoglobulin G binding protein G	[U-15N; U-13C]	3.5 mM
2	sodium phosphate		50 mM
3	sodium azide		0.02 %
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
6	mmunoglobulin G binding protein G	[U-15N]	2.89 mM
7	sodium phosphate		50 mM
8	sodium azide		0.02 %
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
11	mmunoglobulin G binding protein G	[U-15N; U-13C]	3.5 mM
12	sodium phosphate		50 mM
13	sodium azide		0.02 %
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
15	mmunoglobulin G binding protein G		0.7 mM
16	mmunoglobulin G binding protein G	[U-15N; U-13C]	0.7 mM
17	sodium phosphate		50 mM
18	sodium azide		0.02 %
19	D2O		100 %

4 4D 13C-separated NOESY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
1	mmunoglobulin G binding protein G	[U-15N; U-13C]	3.5 mM
2	sodium phosphate		50 mM
3	sodium azide		0.02 %
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
6	mmunoglobulin G binding protein G	[U-15N]	2.89 mM
7	sodium phosphate		50 mM
8	sodium azide		0.02 %
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
11	mmunoglobulin G binding protein G	[U-15N; U-13C]	3.5 mM
12	sodium phosphate		50 mM
13	sodium azide		0.02 %
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
15	mmunoglobulin G binding protein G		0.7 mM
16	mmunoglobulin G binding protein G	[U-15N; U-13C]	0.7 mM
17	sodium phosphate		50 mM
18	sodium azide		0.02 %
19	D2O		100 %

5 4D 13C/15N-separated NOESY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
1	mmunoglobulin G binding protein G	[U-15N; U-13C]	3.5 mM
2	sodium phosphate		50 mM
3	sodium azide		0.02 %
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
6	mmunoglobulin G binding protein G	[U-15N]	2.89 mM
7	sodium phosphate		50 mM
8	sodium azide		0.02 %
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
11	mmunoglobulin G binding protein G	[U-15N; U-13C]	3.5 mM
12	sodium phosphate		50 mM
13	sodium azide		0.02 %
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
15	mmunoglobulin G binding protein G		0.7 mM
16	mmunoglobulin G binding protein G	[U-15N; U-13C]	0.7 mM
17	sodium phosphate		50 mM
18	sodium azide		0.02 %
19	D2O		100 %

6 3D 12C-filtered 13C-separated NOESY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
1	mmunoglobulin G binding protein G	[U-15N; U-13C]	3.5 mM
2	sodium phosphate		50 mM
3	sodium azide		0.02 %
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
6	mmunoglobulin G binding protein G	[U-15N]	2.89 mM
7	sodium phosphate		50 mM
8	sodium azide		0.02 %
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
11	mmunoglobulin G binding protein G	[U-15N; U-13C]	3.5 mM
12	sodium phosphate		50 mM
13	sodium azide		0.02 %
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 313 K, pH 5.45

#	Name	Isotope labeling	Concentration
15	mmunoglobulin G binding protein G		0.7 mM
16	mmunoglobulin G binding protein G	[U-15N; U-13C]	0.7 mM
17	sodium phosphate		50 mM
18	sodium azide		0.02 %
19	D2O		100 %

nullKeywords channel, strand-exchanged tetramer

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.821 seconds)

BMRB: 5654

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Immunoglobulin G binding protein G, : 1 : 1 : 1 : 102 entities Detail

Interaction partners 1. Immunoglobulin G binding protein G, : 1 interactors Detail

Experiments performed 6 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4