BMRB: 5655

U80G U6 ISL RNA Chemical Shifts

Authors

Sashital, D.G., Allmann, A.M., Van Doren, S., Butcher, S.E.

Assembly

U80G U6 ISL RNA

Entity

1. U80G U6 ISL RNA (polymer, Thiol state: not present), 24 monomers, 7770.625 Da Detail

GGUUCCCCUG CAUAAGGAGG AACC

Formula weight

7770.625 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.7 %, Completeness (bb): 87.5 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	88.7 % (339 of 382)	92.4 % (194 of 210)	85.7 % (138 of 161)	63.6 % (7 of 11)
Suger, PO4	87.5 % (231 of 264)	93.1 % (134 of 144)	80.8 % (97 of 120)
Nucleobase	91.5 % (108 of 118)	90.9 % (60 of 66)	100.0 % (41 of 41)	63.6 % (7 of 11)
Aromatic	93.3 % (97 of 104)	94.2 % (49 of 52)	100.0 % (41 of 41)	63.6 % (7 of 11)

1. U6 ISL RNA

GGUUCCCCUG CAUAAGGAGG AACC

Show sample condition

Sample

Temperature 303 (±0.5) K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	U6 ISL RNA	[U-13C; U-15N]		1.5 mM

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Release date

2003-02-24

Citation

Structural basis for a lethal mutation in U6 RNA
Sashital, D.G., Allmann, A.M., Van Doren, S.R., Butcher, S.E.
Biochemistry (2003), 42, 1470-1477, PubMed 12578359 , DOI 10.1021/bi027137h , Abstract

Related entities 1. U80G U6 ISL RNA, : 1 : 9 : 1 entities Detail

Experiments performed 1 experiments Detail