BMRB: 5659

1H, 13C and 15N Backbone Assignments for the Human Rhinovirus-14 3C Protease

Authors

Bjorndahl, T.C., Monzavi, H., Wishart, D.S.

Assembly

Human Rhinovirus-14 3C Protease monomer

Entity

1. Human Rhinovirus-14 3C Protease monomer (polymer, Thiol state: all free), 182 monomers, 19996.65 Da Detail

GPNTEFALSL LRKNIMTITT SKGEFTGLGI HDRVCVIPTH AQPGDDVLVN GQKIRVKDKY KLVDPENINL ELTVLTLDRN EKFRDIRGFI SEDLEGVDAT LVVHSNNFTN TILEVGPVTM AGLINLSSTP TNRMIRYDYA TKTGQCGGVL CATGKIFGIH VGGNGRQGFS AQLKKQYFVE KQ

Formula weight

19996.65 Da

Source organism

rhinovirus B14

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.5 %, Completeness: 86.1 %, Completeness (bb): 92.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.1 % (1805 of 2096)	87.2 % (946 of 1085)	84.4 % (689 of 816)	87.2 % (170 of 195)
Backbone	92.3 % (997 of 1080)	93.9 % (354 of 377)	91.5 % (482 of 527)	91.5 % (161 of 176)
Sidechain	81.6 % (962 of 1179)	83.6 % (592 of 708)	79.9 % (361 of 452)	47.4 % (9 of 19)
Aromatic	46.1 % (59 of 128)	85.9 % (55 of 64)	6.3 % (4 of 64)
Methyl	93.9 % (214 of 228)	95.6 % (109 of 114)	92.1 % (105 of 114)

1. HRV14-3C

GPNTEFALSL LRKNIMTITT SKGEFTGLGI HDRVCVIPTH AQPGDDVLVN GQKIRVKDKY KLVDPENINL ELTVLTLDRN EKFRDIRGFI SEDLEGVDAT LVVHSNNFTN TILEVGPVTM AGLINLSSTP TNRMIRYDYA TKTGQCGGVL CATGKIFGIH VGGNGRQGFS AQLKKQYFVE KQ

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	HRV14-3C	[U-15N]		1 mM
2	KH2PO4			20 mM
3	NaCl			0 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	HRV14-3C	[U-15N; U-13C]		1 mM
5	KH2PO4			20 mM
6	NaCl			0 mM

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LACS Plot; CA

Referencing offset: 0.14 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.101329

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LACS Plot; CB

Referencing offset: 0.14 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.101329

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.101329

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LACS Plot; CO

Referencing offset: 0.68 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.101329

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Protein Blocks Logo

Calculated from 20 models in PDB: 2IN2, Strand ID: A Detail

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Release date

2003-02-16

Citation

Backbone 1H, 15N and 13C assignments for the human rhinovirus 3C protease (serotype 14)
Bjorndahl, T.C., Monzavi, H., Wishart, D.S.
J. Biomol. NMR (2003), 26, 85-86, PubMed 12766405 , DOI: , Abstract

Related entities 1. Human Rhinovirus-14 3C Protease monomer, : 1 : 4 : 140 entities Detail

Experiments performed 6 experiments Detail