BMRB: 5664

Full 1H and 15N Chemical Shift Assignments for Oxytetracycline Acyl Carrier Protein

Authors

Findlow, S.C., Winsor, C., Dempsey, C.E., Crosby, J., Simpson, T.J., Crump, M.P.

Assembly

oxytetracycline acyl carrier protein

Entity

1. oxytetracycline acyl carrier protein (polymer, Thiol state: not reported), 95 monomers, 9915.932 Da Detail

MTLLTLSDLL TLLRECAGEE ESIDLGGDVE DVAFDALGYD SLALLNTVGR IERDYGVQLG DDAVEKATTP RALIEMTNAS LTGASPSAGG AARDK

Formula weight

9915.932 Da

Source organism

Streptomyces rimosus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.0 %, Completeness (bb): 97.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	93.0 % (557 of 599)	91.8 % (462 of 503)	99.0 % (95 of 96)
Backbone	97.9 % (285 of 291)	97.5 % (193 of 198)	98.9 % (92 of 93)
Sidechain	88.3 % (272 of 308)	88.2 % (269 of 305)	100.0 % (3 of 3)
Aromatic	100.0 % (13 of 13)	100.0 % (13 of 13)
Methyl	97.0 % (64 of 66)	97.0 % (64 of 66)

1. Acyl Carrier Protein

MTLLTLSDLL TLLRECAGEE ESIDLGGDVE DVAFDALGYD SLALLNTVGR IERDYGVQLG DDAVEKATTP RALIEMTNAS LTGASPSAGG AARDK

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Sample

Temperature 298 (±0.5) K, pH 5.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Acyl Carrier Protein	[U-97% 15N]		1 mM

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Protein Blocks Logo

Calculated from 28 models in PDB: 1NQ4, Strand ID: A Detail

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Release date

2003-08-07

Citation 1

Solution structure and dynamics of oxytetracycline polyketide synthase acyl carrier protein from Streptomyces rimosus
Findlow, S.C., Winsor, C., Simpson, T.J., Crosby, J., Crump, M.P.
Biochemistry (2003), 42, 8423-8433, PubMed 12859187 , DOI 10.1021/bi0342259 , Abstract

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Citation 2

Sequences of the oxytetracycline polyketide synthase-encoding otc genes from Streptomyces rimosus
Kim, E.S., Bibb, M.J., Butler, M.J., Hopwood, D.A., Sherman, D.H.
Gene (1994), 141, 141-142, PubMed 8163168 , DOI: , Abstract

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Entries sharing articles Swiss-Prot: 2 entries Detail

Related entities 1. oxytetracycline acyl carrier protein, : 1 : 1 : 29 entities Detail

Experiments performed 6 experiments Detail