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BMRB: 5668

1NKU

Backbone and side chain 1H, 13C, and 15N chemical shift assignments for Zinc-containing 3-methyladenine DNA glycosylase I (TAG) from Escherichia coli

Authors

Stivers, J.T., Drohat, A.C., Cao, C.

Assembly

3-methyladenine DNA glycosylase I

Entity

1. 3-methyladenine DNA glycosylase I (polymer, Thiol state: free and other bound), 187 monomers, 21113.91 Da Detail

MERCGWVSQD PLYIAYHDNE WGVPETDSKK LFEMICLEGQ QAGLSWITVL KKRENYRACF HQFDPVKVAA MQEEDVERLV QDAGIIRHRG KIQAIIGNAR AYLQMEQNGE PFADFVWSFV NHQPQMTQAT TLSEIPTSTP ASDALSKALK KRGFKFVGTT ICYSFMQACG LVNDHVVGCC CYPGNKP

2. ZN (non-polymer), 65.409 Da

Total weight

21179.318 Da

Max. entity weight

21113.91 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS4:SG	2:ZN1:ZN
2	na	sing	1:HIS17:NE2	2:ZN1:ZN
3	na	sing	1:HIS175:ND1	2:ZN1:ZN
4	na	sing	1:CYS179:SG	2:ZN1:ZN

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 92.8 %, Completeness (bb): 97.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.8 % (2034 of 2191)	93.3 % (1064 of 1140)	93.2 % (791 of 849)	88.6 % (179 of 202)
Backbone	97.7 % (1079 of 1104)	97.6 % (369 of 378)	98.2 % (538 of 548)	96.6 % (172 of 178)
Sidechain	89.2 % (1125 of 1261)	91.2 % (695 of 762)	89.1 % (423 of 475)	29.2 % (7 of 24)
Aromatic	82.5 % (170 of 206)	90.3 % (93 of 103)	74.7 % (74 of 99)	75.0 % (3 of 4)
Methyl	100.0 % (184 of 184)	100.0 % (92 of 92)	100.0 % (92 of 92)

1. 3-methyladenine DNA glycosylase I

Show sample condition

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.29 ppm, Outliers: 6 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.29 ppm, Outliers: 6 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.2 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 1NKU, Strand ID: A Detail

Release date

2003-10-07

Citation

A novel zinc snap motif conveys structural stability to 3-methyladenine DNA glycosylase I
Kwon, K., Cao, C., Stivers, J.T.
J. Biol. Chem. (2003), 278, 19442-19446, PubMed 12654914 , DOI 10.1074/jbc.M300934200 , Abstract

Related entities 1. 3-methyladenine DNA glycosylase I, : 1 : 2 : 47 entities Detail

Interaction partners 1. 3-methyladenine DNA glycosylase I, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 18 experiments Detail

1 13C-edited HSQC

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

2 15N-edited HSQC

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

3 2D NOESY (t = 125 ms) in D2O

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

4 2D TOCSY in D2O

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

5 HNCA

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

6 HNCO

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

7 HCACO

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

8 HNCACB

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

9 CBCA(CO)NH

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

10 HBHA(CO)NH

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

11 HCCH-TOCSY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

12 HCCH-COSY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

13 15N-edited TOCSY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

14 15N-edited NOESY (t = 125 ms)

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

15 13C-edited NOESY aliphatic (t = 125 ms)

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

16 13C-edited NOESY aromatic (t = 125 ms)

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

17 4D 13C,13C-edited NOESY (t = 100 ms)

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

18 2D 1H, 15N LR-HSQC

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

nullKeywords TAG, 3-Methyladenine DNA glycosylase I, helix-hairpin-helix superfamily, DNA repair

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Found 1 Document (1.887 seconds)

BMRB: 5668

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Related entities 1. 3-methyladenine DNA glycosylase I, : 1 : 2 : 47 entities Detail

Interaction partners 1. 3-methyladenine DNA glycosylase I, : 3 interactors Detail

Experiments performed 18 experiments Detail

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