BMRBj - BMREntryMaster

Found 1 Document (1.069 seconds)

BMRB: 5669

1NJ3

Structure and Ubiquitin Interactions of the Conserved NZF Domain of Npl4

Authors

Wang, B., Alam, S.L., Meyer, H.H., Payne, M., Stemmler, T.L., Davis, D.R., Sundquist, W.I.

Assembly

Npl4 Zinc Finger domain

Entity

1. Npl4 Zinc Finger (polymer, Thiol state: all other bound), 31 monomers, 3373.840 Da Detail

GSTSAMWACQ HCTFMNQPGT GHCEMCSLPR T

2. ZN (non-polymer), 65.409 Da

Total weight

3439.249 Da

Max. entity weight

3373.84 Da

Entity Connection

metal coordination 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:CYS9:SG	2:ZN1:ZN
2	metal coordination	sing	1:CYS12:SG	2:ZN1:ZN
3	metal coordination	sing	1:CYS23:SG	2:ZN1:ZN
4	metal coordination	sing	1:CYS26:SG	2:ZN1:ZN

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

Torsion Angle Dynamics and Simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 96.8 %, Completeness: 89.0 %, Completeness (bb): 91.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.0 % (292 of 328)	88.8 % (150 of 169)	88.1 % (111 of 126)	93.9 % (31 of 33)
Backbone	91.8 % (167 of 182)	92.1 % (58 of 63)	91.1 % (82 of 90)	93.1 % (27 of 29)
Sidechain	87.4 % (152 of 174)	86.8 % (92 of 106)	87.5 % (56 of 64)	100.0 % (4 of 4)
Aromatic	73.3 % (22 of 30)	73.3 % (11 of 15)	71.4 % (10 of 14)	100.0 % (1 of 1)
Methyl	100.0 % (16 of 16)	100.0 % (8 of 8)	100.0 % (8 of 8)

1. Npl4 Zinc Finger

GSTSAMWACQ HCTFMNQPGT GHCEMCSLPR T

Show sample condition

Sample

Pressure 1 atm, Temperature 291 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Npl4 Zinc Finger	[U-15N; U-13C]	1 mM
2	sodium phosphate		20 mM
3	NaCl		50 mM
4	BME		5 mM
5	H2O		90 %
6	D2O		10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 1NJ3, Strand ID: A Detail

Release date

2003-06-09

Citation 1

Structure and ubiquitin interactions of the conserved zinc finger domain of Npl4
Wang, B., Alam, S.L., Meyer, H.H., Payne, M., Stemmler, T.L., Davis, D.R., Sundquist, W.I.
J. Biol. Chem. (2003), 278, 20225-20234, PubMed 12644454 , DOI 10.1074/jbc.M300459200 , Abstract

Show more 4 citaions

Citation 2

Direct binding of ubiquitin conjugates by the mammalian p97 adaptor complexes, p47 and Ufd1-Npl4
Meyer, H.H., Wang, Y., Warren, G.
EMBO J. (2002), 21, 5645-5652, PubMed 12411482 , DOI: , Abstract

Citation 3

Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly
Hetzer, M., Meyer, H.H., Walther, T.C., Bilbao-Cortes, D., Warren, G., Mattaj, I.W.
Nat. Cell Biol. (2001), 3, 1086-1091, PubMed 11781570 , DOI 10.1038/ncb1201-1086 , Abstract

Citation 4

The AAA ATPase Cdc48/p97 and its partners transport proteins from the ER into the cytosol
Ye, Y., Meyer, H.H., Rapoport, T.A.
Nature (2001), 414, 652-656, PubMed 11740563 , DOI 10.1038/414652a , Abstract

Citation 5

A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways
Meyer, H.H., Shorter, J.G., Seemann, J., Pappin, D., Warren, G.
EMBO J. (2000), 19, 2181-2192, PubMed 10811609 , DOI 10.1093/emboj/19.10.2181 , Abstract

Hide non-primary 4 citaions

Related entities 1. Npl4 Zinc Finger, : 1 : 1 : 106 entities Detail

Interaction partners 1. Npl4 Zinc Finger, : 3 interactors Detail

More details for 3 interactors identified by 1 citations

Experiments performed 6 experiments Detail

1 HNCACB

Instrument

#1 Model Varian UNITYplus (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 1 atm, Temperature 291 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Npl4 Zinc Finger	[U-15N; U-13C]	1 mM
2	sodium phosphate		20 mM
3	NaCl		50 mM
4	BME		5 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 291 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Npl4 Zinc Finger	[U-95% 15N]		1 mM

2 HN(CA)CO

Instrument

#1 Model Varian UNITYplus (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 1 atm, Temperature 291 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Npl4 Zinc Finger	[U-15N; U-13C]	1 mM
2	sodium phosphate		20 mM
3	NaCl		50 mM
4	BME		5 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 291 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Npl4 Zinc Finger	[U-95% 15N]		1 mM

3 H(CCO)NH-TOCSY

Instrument

#1 Model Varian UNITYplus (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 1 atm, Temperature 291 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Npl4 Zinc Finger	[U-15N; U-13C]	1 mM
2	sodium phosphate		20 mM
3	NaCl		50 mM
4	BME		5 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 291 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Npl4 Zinc Finger	[U-95% 15N]		1 mM

4 (H)C(CO)NH-TOCSY

Instrument

#1 Model Varian UNITYplus (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 1 atm, Temperature 291 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Npl4 Zinc Finger	[U-15N; U-13C]	1 mM
2	sodium phosphate		20 mM
3	NaCl		50 mM
4	BME		5 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 291 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Npl4 Zinc Finger	[U-95% 15N]		1 mM

5 3D 13C-separated NOESY

Instrument

#1 Model Varian UNITYplus (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 1 atm, Temperature 291 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Npl4 Zinc Finger	[U-15N; U-13C]	1 mM
2	sodium phosphate		20 mM
3	NaCl		50 mM
4	BME		5 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 291 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Npl4 Zinc Finger	[U-95% 15N]		1 mM

6 3D 15N-separated NOESY

Instrument

#1 Model Varian UNITYplus (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 1 atm, Temperature 291 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Npl4 Zinc Finger	[U-15N; U-13C]	1 mM
2	sodium phosphate		20 mM
3	NaCl		50 mM
4	BME		5 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 291 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Npl4 Zinc Finger	[U-95% 15N]		1 mM

nullKeywords beta-ribbon, Npl4, NZF domain, rubredoxin knuckle, ubiquitin, zinc-finger

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Found 1 Document (1.069 seconds)

BMRB: 5669

Sample

Related entities 1. Npl4 Zinc Finger, : 1 : 1 : 106 entities Detail

Interaction partners 1. Npl4 Zinc Finger, : 3 interactors Detail

Experiments performed 6 experiments Detail

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