BMRBj - BMREntryMaster

Found 1 Document (1.396 seconds)

BMRB: 5671

1NAJ

Overall structure and sugar dynamics of a DNA dodecamer from homo and heteronuclear dipolar couplings and 31P chemical shift anisotropy

Authors

Zhengrong, W., Delaglio, F., Tjandra, N., Zhurkin, V., Bax, A.

Assembly

5'-D(*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G)-3'

Entity

1. 5'-D(*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G)-3' (polymer, Thiol state: not present), 12 monomers, 3726.364 × 2 Da Detail

CGCGAATTCG CG

Total weight

7452.728 Da

Max. entity weight

3726.364 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts, coupling_constants, RDCs

Chem. Shift Complete1

Sequence coverage: 91.7 %, Completeness: 91.7 %, Completeness (bb): 91.7 % Detail

Polymer type: polydeoxyribonucleotide

	Total	³¹P
All	91.7 % (11 of 12)	91.7 % (11 of 12)
Suger, PO4	91.7 % (11 of 12)	91.7 % (11 of 12)

1. Dodecamer

CGCGAATTCG CG

Show sample condition

Sample #1

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Concentration
1	Dodecamer	0.5 mM
2	sodium phosphate	40 mM
3	NaN3	0.04 %
4	DMPC/DHPC/CTAB (30/10/1)	5 %
5	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Dodecamer	[U-13C; U-15N]	0.5 mM
7	sodium phosphate		40 mM
8	NaN3		0.04 %
9	pf1 phage		20 mg/mL
10	D2O		10 %
11	H2O		90 %

Chem. Shift Complete2

Sequence coverage: 91.7 %, Completeness: 91.7 %, Completeness (bb): 91.7 % Detail

Polymer type: polydeoxyribonucleotide

	Total	³¹P
All	91.7 % (22 of 24)	91.7 % (22 of 24)
Suger, PO4	91.7 % (22 of 24)	91.7 % (22 of 24)

1. Dodecamer

CGCGAATTCG CG

Show sample condition

Sample #1

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Concentration
1	Dodecamer	0.5 mM
2	sodium phosphate	40 mM
3	NaN3	0.04 %
4	DMPC/DHPC/CTAB (30/10/1)	5 %
5	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Dodecamer	[U-13C; U-15N]	0.5 mM
7	sodium phosphate		40 mM
8	NaN3		0.04 %
9	pf1 phage		20 mg/mL
10	D2O		10 %
11	H2O		90 %

Coupling constant

22 J values in 2 lists
Pressure 1 atm, Temperature 308 K, pH 7.0 Detail

RDC

636 RDC values in 2 lists
Field strength (¹H) 800 MHz, Pressure 1 atm, Temperature 308 K, pH 7.0 Detail

Release date

2003-11-10

Citation

Overall structure and sugar dynamics of a DNA dodecamer from homo- and heteronuclear dipolar couplings and 31P chemical shift anisotropy
Wu, Z., Delaglio, F., Tjandra, N., Zhurkin, V.B., Bax, A.
J. Biomol. NMR (2003), 26, 297-315, PubMed 12815257 , Abstract

Related entities 1. 5'-D(CPGPCPGPAPAPTPTPCPGPCPG)-3', : 4 entities Detail

Experiments performed 7 experiments Detail

1 H-31P-HSQC

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Concentration
1	Dodecamer	0.5 mM
2	sodium phosphate	40 mM
3	NaN3	0.04 %
4	DMPC/DHPC/CTAB (30/10/1)	5 %
5	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Dodecamer	[U-13C; U-15N]	0.5 mM
7	sodium phosphate		40 mM
8	NaN3		0.04 %
9	pf1 phage		20 mg/mL
10	D2O		10 %
11	H2O		90 %

2 HP-ctNOESY-DIF COSY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Concentration
1	Dodecamer	0.5 mM
2	sodium phosphate	40 mM
3	NaN3	0.04 %
4	DMPC/DHPC/CTAB (30/10/1)	5 %
5	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Dodecamer	[U-13C; U-15N]	0.5 mM
7	sodium phosphate		40 mM
8	NaN3		0.04 %
9	pf1 phage		20 mg/mL
10	D2O		10 %
11	H2O		90 %

3 COSY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Concentration
1	Dodecamer	0.5 mM
2	sodium phosphate	40 mM
3	NaN3	0.04 %
4	DMPC/DHPC/CTAB (30/10/1)	5 %
5	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Dodecamer	[U-13C; U-15N]	0.5 mM
7	sodium phosphate		40 mM
8	NaN3		0.04 %
9	pf1 phage		20 mg/mL
10	D2O		10 %
11	H2O		90 %

4 2D NOESY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Concentration
1	Dodecamer	0.5 mM
2	sodium phosphate	40 mM
3	NaN3	0.04 %
4	DMPC/DHPC/CTAB (30/10/1)	5 %
5	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Dodecamer	[U-13C; U-15N]	0.5 mM
7	sodium phosphate		40 mM
8	NaN3		0.04 %
9	pf1 phage		20 mg/mL
10	D2O		10 %
11	H2O		90 %

5 13C-HSQC-J-MODULATED

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Concentration
1	Dodecamer	0.5 mM
2	sodium phosphate	40 mM
3	NaN3	0.04 %
4	DMPC/DHPC/CTAB (30/10/1)	5 %
5	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Dodecamer	[U-13C; U-15N]	0.5 mM
7	sodium phosphate		40 mM
8	NaN3		0.04 %
9	pf1 phage		20 mg/mL
10	D2O		10 %
11	H2O		90 %

6 3C-HSQC-F1-COUPLED

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Concentration
1	Dodecamer	0.5 mM
2	sodium phosphate	40 mM
3	NaN3	0.04 %
4	DMPC/DHPC/CTAB (30/10/1)	5 %
5	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Dodecamer	[U-13C; U-15N]	0.5 mM
7	sodium phosphate		40 mM
8	NaN3		0.04 %
9	pf1 phage		20 mg/mL
10	D2O		10 %
11	H2O		90 %

7 15N-HSQC-F1-COUPLED

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Concentration
1	Dodecamer	0.5 mM
2	sodium phosphate	40 mM
3	NaN3	0.04 %
4	DMPC/DHPC/CTAB (30/10/1)	5 %
5	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Dodecamer	[U-13C; U-15N]	0.5 mM
7	sodium phosphate		40 mM
8	NaN3		0.04 %
9	pf1 phage		20 mg/mL
10	D2O		10 %
11	H2O		90 %

nullKeywords Dickerson Dodecamer

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.396 seconds)

BMRB: 5671

Sample #1

Sample #2

Sample #1

Sample #2

Related entities 1. 5'-D(*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G)-3', : 4 entities Detail

Experiments performed 7 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Related entities 1. 5'-D(CPGPCPGPAPAPTPTPCPGPCPG)-3', : 4 entities Detail