BMRB: 5673

NMR Solution Structure of the Glucagon Antagonist [desHis1, desPhe6, Glu9]Glucagon Amide in the Presence of Perdeuterated Dodecylphosphocholine Micelles

Authors

Ying, J., Ahn, J., Jacobsen, N.E., Brown, M.F., Hruby, V.J.

Assembly

Glucagon analogue

Entity

1. Glucagon analogue (polymer, Thiol state: not present), 28 monomers, 3210.469 Da Detail

SQGTTSEYSK YLDSRRAQDF VQWLMNTX

Formula weight

3210.469 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 86.2 %, Completeness (bb): 96.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	86.2 % (144 of 167)	86.2 % (144 of 167)
Backbone	96.4 % (53 of 55)	96.4 % (53 of 55)
Sidechain	81.3 % (91 of 112)	81.3 % (91 of 112)
Aromatic	57.9 % (11 of 19)	57.9 % (11 of 19)
Methyl	90.0 % (9 of 10)	90.0 % (9 of 10)

1. Glucagon analogue

SQGTTSEYSK YLDSRRAQDF VQWLMNTX

Show sample condition

Sample

Pressure 1 atm, Temperature 310 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Glucagon analogue			7 mM
2	sodium phosphate buffer			50 mM
3	dodecylphosphocholine-d38			283 mM
4	sodium azide			1 mM
5	H2O			90 %
6	D2O			10 %

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Calculated from 16 models in PDB: 1NAU, Strand ID: A Detail

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Release date

2008-07-16

Citation

NMR solution structure of the glucagon antagonist [desHis1, desPhe6, Glu9]glucagon amide in the presence of perdeuterated dodecylphosphocholine micelles
Ying, J., Ahn, J., Jacobsen, N.E., Brown, M.F., Hruby, V.J.
Biochemistry (2003), 42, 2825-2835, PubMed 12627948 , DOI 10.1021/bi026629r , Abstract

Related entities 1. Glucagon analogue, : 1 : 147 entities Detail

Interaction partners 1. Glucagon analogue, : 6 interactors Detail

Experiments performed 3 experiments Detail