BMRB: 5676

1H chemical shifts of Pi4, a four disulfide bridged scorpion toxin active on potassium channels

Authors

Guijarro, J.I., M'Barek, S., Olamendi-Portugal, T., Gomez-Lagunas, F., Garnier, D., Rochat, H., Possani, L.D., Sabatier, J.M., Delepierre, M.

Assembly

Pandinus imperator potassium channel blocking toxin 4

Entity

1. Pandinus imperator potassium channel blocking toxin 4 (polymer, Thiol state: all disulfide bound), 38 monomers, 4187.898 Da Detail

IEAIRCGGSR DCYRPCQKRT GCPNAKCINK TCKCYGCS

Formula weight

4187.898 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS27:SG
2	disulfide	sing	1:CYS12:SG	1:CYS32:SG
3	disulfide	sing	1:CYS16:SG	1:CYS34:SG
4	disulfide	sing	1:CYS22:SG	1:CYS37:SG

Source organism

Pandinus imperator

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 96.0 %, Completeness (bb): 97.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	96.0 % (214 of 223)	96.0 % (214 of 223)
Backbone	97.4 % (76 of 78)	97.4 % (76 of 78)
Sidechain	95.2 % (138 of 145)	95.2 % (138 of 145)
Aromatic	100.0 % (8 of 8)	100.0 % (8 of 8)
Methyl	100.0 % (10 of 10)	100.0 % (10 of 10)

1. Pi4 scorpion toxin

IEAIRCGGSR DCYRPCQKRT GCPNAKCINK TCKCYGCS

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±0.5) K, pH 4.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Pi4 scorpion toxin			2.5 mM
2	sodium acetate			5 mM
3	D2O			10 %

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Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 87.7 %, Completeness (bb): 79.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	87.7 % (391 of 446)	87.7 % (391 of 446)
Backbone	79.5 % (124 of 156)	79.5 % (124 of 156)
Sidechain	92.1 % (267 of 290)	92.1 % (267 of 290)
Aromatic	100.0 % (16 of 16)	100.0 % (16 of 16)
Methyl	100.0 % (20 of 20)	100.0 % (20 of 20)

1. Pi4 scorpion toxin

IEAIRCGGSR DCYRPCQKRT GCPNAKCINK TCKCYGCS

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
4	Pi4 scorpion toxin			3.2 mM
5	sodium acetate			5 mM
6	D2O			100 %

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Protein Blocks Logo

Calculated from 10 models in PDB: 1N8M, Strand ID: A Detail

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Release date

2003-01-23

Citation

Synthesis and characterization of Pi4, a scorpion toxin from Pandinus imperator that acts on K+ channels
M'Barek, S., Mosbah, A., Sandoz, G., Fajloun, Z., Olamendi-Portugal, T., Rochat, H., Sampieri, F., Guijarro, J.I., Mansuelle, P., Delepierre, M., De Waard, M., Sabatier, J.M.
Eur. J. Biochem. (2003), 270, 3583-3592, PubMed 12919322 , DOI: , Abstract

Related entities 1. Pandinus imperator potassium channel blocking toxin 4, : 1 : 1 : 133 entities Detail

Experiments performed 3 experiments Detail