BMRB: 5678

1H, 15N, and 13C assigned Chemical shift for HNF-6a

Authors

Sheng, W.

Assembly

HNF6 protein

Entity

1. HNF6 protein (polymer, Thiol state: all free), 162 monomers, 19229.05 Da Detail

MGQMEEINTK EVAQRITTEL KRYSIPQAIF AQRVLCRSQG TLSDLLRNPK PWSKLKSGRE TFRRMWKWLQ EPEFQRMSAL RLAACKRKEQ EHGKDRGNTP KKPRLVFTDV QRRTLHAIFK ENKRPSKELQ ITISQQLGLE LSTVSNFFMN ARRRSLDKWQ DL

Formula weight

19229.05 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 78.4 %, Completeness (bb): 89.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	78.4 % (1594 of 2032)	75.5 % (821 of 1087)	81.7 % (627 of 767)	82.0 % (146 of 178)
Backbone	89.4 % (856 of 958)	89.5 % (289 of 323)	88.1 % (423 of 480)	92.9 % (144 of 155)
Sidechain	70.4 % (866 of 1230)	69.6 % (532 of 764)	74.9 % (332 of 443)	8.7 % (2 of 23)
Aromatic	64.9 % (87 of 134)	76.1 % (51 of 67)	55.6 % (35 of 63)	25.0 % (1 of 4)
Methyl	87.5 % (133 of 152)	86.8 % (66 of 76)	88.2 % (67 of 76)

1. HNF6

MGQMEEINTK EVAQRITTEL KRYSIPQAIF AQRVLCRSQG TLSDLLRNPK PWSKLKSGRE TFRRMWKWLQ EPEFQRMSAL RLAACKRKEQ EHGKDRGNTP KKPRLVFTDV QRRTLHAIFK ENKRPSKELQ ITISQQLGLE LSTVSNFFMN ARRRSLDKWQ DL

Show sample condition

Sample

Temperature 295 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	HNF6	[U-13C; U-15N; U-2H]		1.0 ~ 2.0 mM

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LACS Plot; CA

Referencing offset: 2.57 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 2.57 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.23 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 1.83 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1S7E, Strand ID: A Detail

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Release date

2004-02-12

Citation

Letter to the Editor: 1H, 13C, and 15N resonance assignments of the hepatocyte nuclear factor 6alpha (HNF-6alpha)
Sheng, W., Tan, Y., Liao, X.
J. Biomol. NMR (2004), 28, 401-402

Related entities 1. HNF6 protein, : 1 : 7 : 42 entities Detail