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1H, 13C, and 15N backbone assignments of the pheromone binding protein from the silk moth Antheraea polyphemus (ApolPBP)

Authors

Mohanty, S., Zubkov, S., Campos-Olivas, R.

Assembly

Pheromone Binding Protein from Antheraea polyphemus

Entity

1. Pheromone Binding Protein from Antheraea polyphemus (polymer, Thiol state: all disulfide bound), 142 monomers, 15782.93 Da Detail

SPEIMKNLSN NFGKAMDQCK DELSLPDSVV ADLYNFWKDD YVMTDRLAGC AINCLATKLD VVDPDGNLHH GNAKDFAMKH GADETMAQQL VDIIHGCEKS APPNDDKCMK TIDVAMCFKK EIHKLNWVPN MDLVIGEVLA EV

Formula weight

15782.93 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS19:SG	1:CYS54:SG
2	disulfide	sing	1:CYS50:SG	1:CYS108:SG
3	disulfide	sing	1:CYS97:SG	1:CYS117:SG

Source organism

Antheraea polyphemus

Exptl. method

NMR

Refine. method

torsion angle dynamics simulated annealing, thin layer water refinement

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 91.3 %, Completeness (bb): 97.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.3 % (1477 of 1618)	93.4 % (783 of 838)	87.1 % (548 of 629)	96.7 % (146 of 151)
Backbone	97.4 % (818 of 840)	97.2 % (277 of 285)	97.6 % (409 of 419)	97.1 % (132 of 136)
Sidechain	85.4 % (780 of 913)	90.4 % (500 of 553)	77.7 % (268 of 345)	80.0 % (12 of 15)
Aromatic	38.0 % (38 of 100)	64.0 % (32 of 50)	10.4 % (5 of 48)	50.0 % (1 of 2)
Methyl	86.2 % (131 of 152)	96.1 % (73 of 76)	76.3 % (58 of 76)

1. Pheromone-binding protein from Antheraea polyphemus

SPEIMKNLSN NFGKAMDQCK DELSLPDSVV ADLYNFWKDD YVMTDRLAGC AINCLATKLD VVDPDGNLHH GNAKDFAMKH GADETMAQQL VDIIHGCEKS APPNDDKCMK TIDVAMCFKK EIHKLNWVPN MDLVIGEVLA EV

Show sample condition

Sample

Temperature 308 (±0.2) K, pH 6.33 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Pheromone-binding protein from Antheraea polyphemus	[U-99% 13C; U-99% 15N]		1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1QWV, Strand ID: A Detail

Release date

2003-02-06

Citation

1H, 13C and 15N backbone assignments of the pheromone binding protein from the silk moth Antheraea polyphemus (ApolPBP)
Mohanty, S., Zubkov, S., Campos-Olivas, R.
J. Biomol. NMR (2003), 27, 393-394, PubMed 14512737 , DOI: , Abstract

Related entities 1. Pheromone Binding Protein from Antheraea polyphemus, : 1 : 3 : 45 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC