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Found 1 Document (1.776 seconds)

BMRB: 5695

1NYN

Solution Structure of the Product of the S. Cerevisiae YHR087W Gene, Northeast Structural Genomics Consortium target YTYst425

Authors

Cort, J.R., Yee, A., Arrowsmith, C.H., Kennedy, M.A.

Assembly

YHR087W gene product

Entity

1. YHR087W gene product (polymer, Thiol state: not present), 131 monomers, 14172.60 Da Detail

MGSSHHHHHH SSGLVPRGSH MSTVTKYFYK GENTDLIVFA ASEELVDEYL KNPSIGKLSE VVELFEVFTP QDGRGAEGEL GAASKAQVEN EFGKGKKIEE VIDLILRNGK PNSTTSSLKT KGGNAGTKAY N

Formula weight

14172.6 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 74.8 %, Completeness: 65.9 %, Completeness (bb): 68.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	65.9 % (970 of 1472)	63.0 % (481 of 763)	68.9 % (395 of 573)	69.1 % (94 of 136)
Backbone	68.1 % (530 of 778)	65.9 % (180 of 273)	69.6 % (263 of 378)	68.5 % (87 of 127)
Sidechain	64.9 % (526 of 810)	61.4 % (301 of 490)	70.1 % (218 of 311)	77.8 % (7 of 9)
Aromatic	45.5 % (50 of 110)	45.5 % (25 of 55)	45.5 % (25 of 55)
Methyl	79.7 % (102 of 128)	79.7 % (51 of 64)	79.7 % (51 of 64)

1. YHR087W

MGSSHHHHHH SSGLVPRGSH MSTVTKYFYK GENTDLIVFA ASEELVDEYL KNPSIGKLSE VVELFEVFTP QDGRGAEGEL GAASKAQVEN EFGKGKKIEE VIDLILRNGK PNSTTSSLKT KGGNAGTKAY N

Show sample condition

Sample

Temperature 298 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	YHR087W	[U-13C; U-15N]	1 mM
2	sodium acetate		10 mM
3	sodium chloride		300 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.45 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.45 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.02 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.19 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1NYN, Strand ID: A Detail

Coupling constant

60 J values in 1 lists
Temperature 298 (±1) K, pH 5.0 (±0.2) Detail

Release date

2003-04-29

Citation

Solution Structure of the Product of the S. Cerevisiae YHR087W Gene
Cort, J.R., Yee, A., Arrowsmith, C.H., Kennedy, M.A.

Related entities 1. YHR087W gene product, : 1 : 2 : 3 entities Detail

Interaction partners 1. YHR087W gene product, : 10 interactors Detail

More details for 10 interactors identified by 4 citations

Experiments performed 7 experiments Detail

1 HNCACB

Instrument

#1 Model Varian Inova (750 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Unity (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	YHR087W	[U-13C; U-15N]	1 mM
2	sodium acetate		10 mM
3	sodium chloride		300 mM

Sample #2

Temperature 298 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Concentration
4	YHR087W	[U-10% 13C; 99%-15N]	1 mM
5	sodium acetate		10 mM
6	sodium chloride		300 mM

2 CBCA(CO)NH

Instrument

#1 Model Varian Inova (750 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Unity (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	YHR087W	[U-13C; U-15N]	1 mM
2	sodium acetate		10 mM
3	sodium chloride		300 mM

Sample #2

Temperature 298 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Concentration
4	YHR087W	[U-10% 13C; 99%-15N]	1 mM
5	sodium acetate		10 mM
6	sodium chloride		300 mM

3 HNCO

Instrument

#1 Model Varian Inova (750 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Unity (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	YHR087W	[U-13C; U-15N]	1 mM
2	sodium acetate		10 mM
3	sodium chloride		300 mM

Sample #2

Temperature 298 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Concentration
4	YHR087W	[U-10% 13C; 99%-15N]	1 mM
5	sodium acetate		10 mM
6	sodium chloride		300 mM

4 HCCH-TOCSY

Instrument

#1 Model Varian Inova (750 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Unity (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	YHR087W	[U-13C; U-15N]	1 mM
2	sodium acetate		10 mM
3	sodium chloride		300 mM

Sample #2

Temperature 298 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Concentration
4	YHR087W	[U-10% 13C; 99%-15N]	1 mM
5	sodium acetate		10 mM
6	sodium chloride		300 mM

5 HCC-TOCSY-NNH

Instrument

#1 Model Varian Inova (750 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Unity (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	YHR087W	[U-13C; U-15N]	1 mM
2	sodium acetate		10 mM
3	sodium chloride		300 mM

Sample #2

Temperature 298 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Concentration
4	YHR087W	[U-10% 13C; 99%-15N]	1 mM
5	sodium acetate		10 mM
6	sodium chloride		300 mM

6 CCC-TOCSY-NNH

Instrument

#1 Model Varian Inova (750 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Unity (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	YHR087W	[U-13C; U-15N]	1 mM
2	sodium acetate		10 mM
3	sodium chloride		300 mM

Sample #2

Temperature 298 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Concentration
4	YHR087W	[U-10% 13C; 99%-15N]	1 mM
5	sodium acetate		10 mM
6	sodium chloride		300 mM

7 CBCACOCAHA

Instrument

#1 Model Varian Inova (750 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Unity (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	YHR087W	[U-13C; U-15N]	1 mM
2	sodium acetate		10 mM
3	sodium chloride		300 mM

Sample #2

Temperature 298 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Concentration
4	YHR087W	[U-10% 13C; 99%-15N]	1 mM
5	sodium acetate		10 mM
6	sodium chloride		300 mM

nullKeywords YHR087W, YHO7 YEAST

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Found 1 Document (1.776 seconds)

BMRB: 5695

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. YHR087W gene product, : 1 : 2 : 3 entities Detail

Interaction partners 1. YHR087W gene product, : 10 interactors Detail

Experiments performed 7 experiments Detail

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