BMRB: 5698

Backbone and Side-chain 1H, and 15N Chemical Shift Assignments for TcPABC

Authors

Siddiqui, N., Kozlov, G., D'Orso, I., Trempe, J., Gehring, K.

Assembly

C-terminal domain of poly(A)-binding protein

Entity

1. C-terminal domain of poly(A)-binding protein (polymer, Thiol state: not present), 85 monomers, 9090.288 Da Detail

GSSLASQGQN LSTVLANLTP EQQKNVLGER LYNHIVAINP AAAAKVTGML LEMDNGEILN LLDTPGLLDA KVQEALEVLN RHMNV

Formula weight

9090.288 Da

Source organism

Trypanosoma cruzi

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 95.3 %, Completeness: 75.3 %, Completeness (bb): 95.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	75.3 % (441 of 586)	73.9 % (362 of 490)	82.3 % (79 of 96)
Backbone	95.3 % (243 of 255)	94.8 % (164 of 173)	96.3 % (79 of 82)
Sidechain	59.8 % (198 of 331)	62.5 % (198 of 317)	0.0 % (0 of 14)
Aromatic	62.5 % (5 of 8)	62.5 % (5 of 8)
Methyl	77.8 % (49 of 63)	77.8 % (49 of 63)

1. C-terminal domain of PABP

GSSLASQGQN LSTVLANLTP EQQKNVLGER LYNHIVAINP AAAAKVTGML LEMDNGEILN LLDTPGLLDA KVQEALEVLN RHMNV

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Sample

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	C-terminal domain of PABP	[U-15N]		2 mM
2	sodium phosphate			50 mM
3	sodium chloride			100 mM
4	sodium azide			1 mM

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Calculated from 20 models in PDB: 1NMR, Strand ID: A Detail

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Coupling constant

74 J values in 1 lists
Temperature 303 (±1) K, pH 6.3 (±0.1) Detail

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Temperature 303 (±1) K, pH 6.3 (±0.1)

List #1 coupling_constant_1

Release date

2003-09-11

Citation

Solution structure of the C-terminal domain from poly(A)-binding protein in Trypanosoma cruzi: a vegetal PABC domain
Siddiqui, N., Kozlov, G., D'Orso, I., Trempe, J., Gehring, K.
Protein Sci. (2003), 12, 1925-1933, PubMed 12930992 , DOI 10.1110/ps.0390103 , Abstract

Related entities 1. C-terminal domain of poly(A)-binding protein, : 1 : 1 : 115 entities Detail

Experiments performed 6 experiments Detail