BMRB: 5710

Structure and interactions of Mth11: A homologue of human RNase P protein Rpp29

Authors

Boomershine, W.P., McElroy, C., Tsai, H., Gopalan, V., Foster, M.P.

Assembly

Mth11 free in solution

Entity

1. Mth11 free in solution (polymer, Thiol state: not present), 97 monomers, 11134.78 Da Detail

GSHMLITPRN IFRHELIGLS VRIARSVHRD IQGISGRVVD ETRNTLRIEM DDGREITVPK GIAVFHFRTP QGELVEIDGR ALVARPEERI KKKFRKP

Formula weight

11134.78 Da

Source organism

Methanothermobacter thermautotrophicus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.8 %, Completeness: 74.4 %, Completeness (bb): 76.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	74.4 % (862 of 1158)	77.3 % (473 of 612)	67.6 % (304 of 450)	88.5 % (85 of 96)
Backbone	76.4 % (437 of 572)	88.3 % (174 of 197)	64.3 % (182 of 283)	88.0 % (81 of 92)
Sidechain	73.6 % (497 of 675)	70.8 % (294 of 415)	77.7 % (199 of 256)	100.0 % (4 of 4)
Aromatic	21.4 % (12 of 56)	42.9 % (12 of 28)	0.0 % (0 of 28)
Methyl	94.9 % (112 of 118)	96.6 % (57 of 59)	93.2 % (55 of 59)

1. Mth11

GSHMLITPRN IFRHELIGLS VRIARSVHRD IQGISGRVVD ETRNTLRIEM DDGREITVPK GIAVFHFRTP QGELVEIDGR ALVARPEERI KKKFRKP

Show sample condition

Sample

Temperature 299 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Mth11	[U-98% 13C; U-98% 15N]		1.0 ~ 1.5 mM

Hide sample codition

LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 22 models in PDB: 1OQK, Strand ID: A Detail

---

Release date

2004-02-11

Citation

Structure of Mth11/Mth Rpp29, an essential protein subunit of archaeal and eukaryotic RNase P
Boomershine, W.P., McElroy, C.A., Tsai, H., Wilson, R.C., Gopalan, V., Foster, M.P.
Proc. Natl. Acad. Sci. U. S. A. (2003), 100, 15398-15403, PubMed 14673079 , DOI 10.1073/pnas.2535887100 , Abstract

Related entities 1. Mth11 free in solution, : 1 : 54 entities Detail

Interaction partners 1. Mth11 free in solution, : 1 interactors Detail