BMRB: 5714

1H Chemical shift assignments of the major conformation of a 11-mer DNA duplex containing an AG Mismatch

Authors

Sanchez, A.M., Volk, D.E., Gorenstein, D.G., Lloyd, R.STEPHEN.

Assembly

Synthetic DNA duplex with an AG mismatch

Entity

1. DNA Duplex (polymer, Thiol state: not present), 11 monomers, 3403.155 Da Detail

GCTTCAGTCG T

2. DNA Duplex (polymer, Thiol state: not present), 11 monomers, 3455.210 Da Detail

ACGACGGAAG C

Total weight

6858.365 Da

Max. entity weight

3455.21 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 50.0 %, Completeness: 49.1 %, Completeness (bb): 48.7 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	49.1 % (105 of 214)	49.1 % (105 of 214)
Suger, PO4	48.7 % (75 of 154)	48.7 % (75 of 154)
Nucleobase	50.0 % (30 of 60)	50.0 % (30 of 60)
Aromatic	45.5 % (20 of 44)	45.5 % (20 of 44)
Methyl	100.0 % (4 of 4)	100.0 % (4 of 4)

1. DNA Duplex

GCTTCAGTCG T

2. DNA Duplex

ACGACGGAAG C

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	DNA Duplex			1.0 mM
2	DNA Duplex			1.0 mM
3	Na Phosphate			25.0 mM
4	NaCl			50.0 mM
5	EDTA			1.0 mM

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Chem. Shift Complete2

Sequence coverage: 50.0 %, Completeness: 48.4 %, Completeness (bb): 48.4 % Detail

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• NMR-STAR v3.1 (partial)
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• CSV
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Polymer type: polydeoxyribonucleotide

	Total	1H
All	48.4 % (207 of 428)	48.4 % (207 of 428)
Suger, PO4	48.4 % (149 of 308)	48.4 % (149 of 308)
Nucleobase	48.3 % (58 of 120)	48.3 % (58 of 120)
Aromatic	47.7 % (42 of 88)	47.7 % (42 of 88)
Methyl	50.0 % (4 of 8)	50.0 % (4 of 8)

1. DNA Duplex

GCTTCAGTCG T

2. DNA Duplex

ACGACGGAAG C

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	DNA Duplex			1.0 mM
2	DNA Duplex			1.0 mM
3	Na Phosphate			25.0 mM
4	NaCl			50.0 mM
5	EDTA			1.0 mM

Hide sample codition

Release date

2003-09-14

Citation

Initiation of repair of A/G mismatches is modulated by sequence context
Sanchez, A.M., Volk, D.E., Gorenstein, D.G., Lloyd, R.STEPHEN.
DNA Repair (Amst). (2003), 2, 863-878, PubMed 12893083 , DOI 10.1016/s1568-7864(03)00067-3 , Abstract

Experiments performed 1 experiments Detail